Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit

Catalog #: QLB00B
12 Citations Datasheet / COA / SDS

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QLB00B

Control Products Available

QC190: QuantiGlo Immunoassay Control Set 732 for Human IL-1 beta

Human IL-1 beta/IL-1F2 Chemiluminescent ELISA Standard Curve

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All IL-1 beta/IL-1F2 ELISAs

Product Details

Citations (12)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit Summary

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)

Sensitivity

0.55 pg/mL

Assay Range

1.4 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)

Specificity

Natural and recombinant human IL-1 beta. This assay also recognizes natural and recombinant rhesus macaque IL-1 beta. This assay cross-reacts 15.4% with recombinant Human Pro-IL-1 beta, 1.0% with recombinant Rat IL-1 beta, 0.6% with recombinant Cotton Rat IL-1 beta.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The QuantiGlo Human IL-1 beta Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant protein. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.

Precision

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

	Intra-Assay Precision				Inter-Assay Precision
Sample	1	2	3	4	1	2	3	4
n	20	20	20	20	20	20	20	20
Mean (pg/mL)	7.96	103	638	4.18	9.86	136	695	5.13
Standard Deviation	0.31	5	30	0.16	0.71	9	38	0.4
CV%	3.9	4.9	4.7	3.8	7.2	6.6	5.5	7.8

Recovery

The recovery of IL-1 beta spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	106	100-115
Citrate Plasma (n=4)	95	90-101
EDTA Plasma (n=4)	94	88-107
Heparin Plasma (n=4)	93	87-103
Serum (n=4)	96	87-102

Linearity

To assess the linearity of the assay, four samples containing or spiked with high concentrations of IL-1 beta in various matrices were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human IL-1 beta/IL-1F2 Chemiluminescent ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:

Interleukin 1 beta

Entrez Gene IDs:

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names:

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Related Research Areas

Citations for Human IL-1 beta/IL-1F2 QuantiGlo ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Intestinal Dysbiosis, Tight Junction Proteins, and Inflammation in Rheumatoid Arthritis Patients: A Cross-Sectional Study
Authors: Mucientes, A;Lisbona-Montañez, JM;Mena-Vázquez, N;Ruiz-Limón, P;Manrique-Arija, S;García-Studer, A;Ortiz-Márquez, F;Fernández-Nebro, A;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK
Authors: C Ge, J Tan, X Dai, Q Kuang, S Zhong, L Lai, C Yi, Y Sun, J Luo, C Zhang, L Zhu, B Wang, M Xu
Oncogene, 2022-10-08;13(1):5945.
Species: Human
Sample Types: Cell Culture Supernates
Procyanidinï¿½B2 inhibits lipopolysaccharide?induced apoptosis by suppressing the Bcl?2/Bax and NF?&kappaB signalling pathways in human umbilical vein endothelial cells
Authors: DQ Song, J Liu, F Wang, XF Li, MH Liu, Z Zhang, SS Cao, X Jiang
Molecular Medicine Reports, 2021-02-12;23(4):1.
Species: Human
Sample Types: Cell Culture Supernates
Polarized Cytokine Release Triggered by P2X7 Receptor from Retinal Pigmented Epithelial Cells Dependent on Calcium Influx
Authors: X Shao, S Guha, W Lu, KE Campagno, JM Beckel, JA Mills, W Yang, CH Mitchell
Cells, 2020-11-24;9(12):.
Species: Human
Sample Types: Cell Culture Supernates
miR-98 Modulates Cytokine Production from Human PBMCs in Systemic Lupus Erythematosus by Targeting IL-6 mRNA
Authors: S Yuan, C Tang, D Chen, F Li, M Huang, J Ye, Z He, W Li, Y Chen, X Lin, X Wang, X Cai
J Immunol Res, 2019-12-01;2019(0):9827574.
Species: Human
Sample Types: cell culture
Evaluation of psychological stress in confined environments using salivary, skin, and facial image parameters
Authors: M Egawa, S Haze, Y Gozu, J Hosoi, T Onodera, Y Tojo, M Katsuyama, Y Hara, C Katagiri, N Inoue, S Furukawa, G Suzuki
Sci Rep, 2018-05-29;8(1):8264.
Species: Human
Sample Types: Whole Tissue
Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
Oncotarget, 2017-09-15;8(54):92134-92142.
Species: Human
Sample Types: Cell Culture Supernates
Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
Authors: Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M
Mol Cell Endocrinol, 2012-03-16;361(1):24-30.
Species: Human
Sample Types: Cell Culture Supernates
Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study.
Authors: Fichorova RN, Richardson-Harman N, Alfano M, Belec L, Carbonneil C, Chen S, Cosentino L, Curtis K, Dezzutti CS, Donoval B, Doncel GF, Donaghay M, Grivel JC, Guzman E, Hayes M, Herold B, Hillier S, Lackman-Smith C, Landay A, Margolis L, Mayer KH, Pasicznyk JM, Pallansch-Cokonis M, Poli G, Reichelderfer P, Roberts P, Rodriguez I, Saidi H, Sassi RR, Shattock R, Cummins JE
Anal. Chem., 2008-05-17;80(12):4741-51.
Species: Human
Sample Types: Reference Standard
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
Br. J. Pharmacol., 2007-03-12;151(1):115-27.
Species: Human
Sample Types: Cell Lysates

Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients.
Authors: Dahaba AA, Elawady GA, Rehak PH, List WF
Anaesth Intensive Care, 2002-06-01;30(3):269-74.
Species: Human
Sample Types: Plasma

Rapid secretion of interleukin-1beta by microvesicle shedding.
Authors: MacKenzie A, Wilson HL, Kiss-Toth E, Dower SK, North RA, Surprenant A
Immunity, 2001-11-01;15(5):825-35.
Species: Human
Sample Types: Cell Culture Supernates