Human IL-1 beta /IL-1F2 Antibody Summary
aa 117-269
Accession # P01584
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201). The ND50 is typically 0.001-0.003 µg/mL.
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Detection of Human IL-1 beta/IL-1F2 by Western Blot Hsp27 modulates IL-1 beta production in monocytes.(a) Immunoblot analysis of Hsp27 protein expression in classical (CL) and non-classical (NC) monocytes (left), and relative band densities from 2 combined experiments (right), with GAPDH as loading control. (b) Representative immunoblot of Hsp27 and pro-IL-1 beta (p31) in lysates from siRNA-transfected total monocytes for si-control (scrambled control siRNA) or si-Hsp27 (siRNA to Hsp27) either untreated or stimulated with LPS for 3 h. Data in (a) and (b) are cropped images and the corresponding full-length images are presentated in Supplementary Fig. 3. (c) Expression of IL-1 beta mRNA in siRNA-transfected total monocytes (n = 3). (d) Mature IL-1 beta release measured by ELISA of supernatants from siRNA-transfected total monocytes upon LPS stimulation in the presence or absence of BzATP (n = 4). (e) Fold change in IL-1 beta production by LPS and BzATP-treated total monocytes transfected with si-Hsp27, with respect to si-control. Data plotted are mean ± SEM, student t-test (a and b) and two-way ANOVA with Tukey’s multiple comparison test (c), *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27976724), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Western Blot Non-classical monocytes secrete less mature IL-1 beta compared to other monocyte subsets following LPS and BzATP stimulation.(a) IL-1 beta ELISA on supernatants from monocyte subsets either untreated or stimulated with LPS for 24 h in the presence or absence of the P2X7 antagonist, A438079, followed by a secondary stimulation by BzATP in some conditions as indicated. Data plotted are mean ± SEM; n = 8. ****p < 0.0001 ***p < 0.001. ##p < 0.01 and #p < 0.05 is comparison to the respective classical and intermediate subsets treated with LPS + BzATP. Statistics are calculated based on Two-way ANOVA with Tukey’s multiple comparison test (*p < 0.05). (b) Representative cropped immunoblot images for mature 17 kDa (p17) IL-1 beta in culture supernatants of monocyte subsets stimulated as described in (a). The samples were derived from the same experiment and were ran on the same gel. Full-length images are shown in Supplementary Fig. 3. (c) YoPro-1 dye uptake was measured by flow cytometry to reflect level of P2X7R activity, and is shown as the fold change of BzATP-induced YoPro-1 uptake normalized to the respective untreated subset uptake. Data plotted are mean ± SEM; n = 6 based on one-way ANOVA with Tukey’s multiple comparison test. CL: classical, INT: intermediate, NC: non-classical. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27976724), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Rabbit IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence Immunohistochemistry for CD3, IL-1 beta and CD79 expression.Some expression for these markers were found in the CS and CSVT groups at 21 days (positive signal by brown staining). The same overall expression to the materials was seen at the other time points. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0136514), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Human IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A Novel Circular RNA Mediates Pyroptosis of Diabetic Cardiomyopathy by Functioning as a Competing Endogenous RNA
Authors: Fan Yang, Anqi Li, Ying Qin, Hui Che, Yueqiu Wang, Jie Lv et al.
Molecular Therapy - Nucleic Acids
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Caspase-1 Promiscuity Is Counterbalanced by Rapid Inactivation of Processed Enzyme*
Authors: John G. Walsh, Susan E. Logue, Alexander U. Lüthi, Seamus J. Martin
Journal of Biological Chemistry
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Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens
Authors: Vijayashree Mysore, Xavier Cullere, Matthew L. Settles, Xinge Ji, Michael W. Kattan, Michaël Desjardins et al.
Med (N Y)
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NOX4-dependent fatty acid oxidation promotes NLRP3 inflammasome activation in macrophages
Authors: Jong-Seok Moon, Kiichi Nakahira, Kuei-Pin Chung, Gina M. DeNicola, Michael Jakun Koo, Maria A. Pabón et al.
Nature Medicine
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NLRP3 and downstream cytokine expression elevated in the monocytes of patients with coronary artery disease
Authors: Lili Wang, Peng Qu, Jian Zhao, Yeting Chang
Archives of Medical Science
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Cholesterol crystals induce inflammatory cytokines expression in a human retinal pigment epithelium cell line by activating the NF-kappa B pathway
Authors: Yijun Hu, Haijiang Lin, Bernard Dib, Alp Atik, Peggy Bouzika, Christopher Lin et al.
Discov Med
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Temporal interleukin-1beta secretion from primary human peripheral blood monocytes by P2X7-independent and P2X7-dependent mechanisms
Authors: Jon R. Ward, Peter W. West, Mark P. Ariaans, Lisa C. Parker, Sheila E. Francis, David C. Crossman et al.
Journal of Biological Chemistry
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Cystathionine-beta -synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activation
Authors: Haoran Zhu, Keefe T Chan, Xinran Huang, Carmelo Cerra, Shaun Blake, Anna S Trigos et al.
eLife
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Functional analysis of human circulating immune cells based on high-dimensional mass cytometry
Authors: Xiuxing Liu, Jianjie Lv, Huishi Wang, Yingfeng Zheng, Wenru Su
STAR Protocols
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Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor Prognosis
Authors: Rajia Bahri, Orsolya Kiss, Ian Prise, Karen M. Garcia-Rodriguez, Haris Atmoko, Julia M. Martínez-Gómez et al.
Frontiers in Immunology
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Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis
Authors: Takashi Maehara, Naoki Kaneko, Cory A. Perugino, Hamid Mattoo, Jesper Kers,, Hugues Allard-Chamard et al.
Journal of Clinical Investigation
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Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes
Authors: Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al.
The Journal of Immunology
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Aldara activates TLR7-independent immune defence
Authors: Anne Walter, Matthias Schäfer, Virginia Cecconi, Claudia Matter, Mirjana Urosevic-Maiwald, Benedetta Belloni et al.
Nature Communications
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CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer
Authors: Yu-Chuan Lin, Chun-Hung Hua, Hsin-Man Lu, Shi-Wei Huang, Yeh Chen, Ming-Hsui Tsai et al.
iScience
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HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers
Authors: Xiulei Mo, Cong Tang, Qiankun Niu, Tingxuan Ma, Yuhong Du, Haian Fu
Cell Chemical Biology
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A therapeutic strategy of parthenolide in improving imiquimod-induced psoriasis-like skin inflammation targeting IL-36/NETs through skin transdermal therapeutic system
Authors: Zhan, ZY;Zhang, ZH;Sun, RH;Wu, YL;Nan, JX;Lian, LH;
International immunopharmacology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Mass Cytometry -
Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation
Authors: Bass, AR;Egan, MS;Alexander-Floyd, J;Lopes Fischer, N;Doerner, J;Shin, S;
mBio
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Yersinia deploys type III-secreted effectors to evade caspase-4 inflammasome activation in human cells
Authors: Zhang, J;Brodsky, IE;Shin, S;
mBio
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Discovery of senolytics using machine learning
Authors: Smer-Barreto, V;Quintanilla, A;Elliott, RJR;Dawson, JC;Sun, J;Campa, VM;Lorente-Macías, Á;Unciti-Broceta, A;Carragher, NO;Acosta, JC;Oyarzún, DA;
Nature communications
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Yersinia Type III-Secreted Effectors Subvert Caspase-4-dependent Inflammasome Activation in Human Cells
Authors: J Zhang, IE Brodsky, S Shin
bioRxiv : the preprint server for biology, 2023-01-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1beta to downregulate PPARalpha and FOXO3
Authors: Y Li, Y Zhu, S Feng, Y Ishida, TP Chiu, T Saito, S Wang, DK Ann, JJ Ou
Cell Reports, 2022-01-25;38(4):110284.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Gasdermin D mediates the maturation and release of IL-1&alpha downstream of inflammasomes
Authors: K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda
Cell Reports, 2021-03-23;34(12):108887.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
Applications: Western Blot -
Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model
Authors: S Lichtenegg, J Stiehler, S Saiger, A Zauner, B Kleinhappl, C Bernecker, P Schlenke, GE Wagner, K Krause, M Gastager, I Steinmetz
PLoS Negl Trop Dis, 2020-11-02;14(11):e0008840.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells
Authors: VM Abrahams, Z Tang, G Mor, S Guller
J Reprod Immunol, 2020-10-03;142(0):103214.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair
Authors: H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper
J Neuroinflammation, 2020-09-09;17(1):267.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Autoimmune inner ear disease patient-associated 28-kDa proinflammatory IL-1beta fragment results from caspase-7-mediated cleavage in vitro
Authors: S Pathak, A Vambutas
JCI Insight, 2020-02-13;5(3):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling
Authors: R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke
Nat Commun, 2019-11-01;10(1):5016.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus
Authors: K Vogel, M Pierau, A Arra, K Lampe, D Schlueter, C Arens, MC Brunner-We
Sci Rep, 2018-11-15;8(1):16904.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing
Authors: DM Clancy, GP Sullivan, HBT Moran, CM Henry, EP Reeves, NG McElvaney, EC Lavelle, SJ Martin
Cell Rep, 2018-03-13;22(11):2937-2950.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1?
Authors: SEJ Chambers, CL O'Neill, J Guduric-Fu, KJ McLoughlin, A Liew, AM Egan, T O'Brien, AW Stitt, RJ Medina
Stem Cells, 2018-03-09;36(6):834-843.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot -
Galectin-3 is an amplifier of the interleukin-1?-mediated inflammatory response in corneal keratinocytes
Authors: Y Uchino, AM Woodward, J Mauris, K Peterson, P Verma, UJ Nilsson, J Rajaiya, P Argüeso
Immunology, 2018-02-15;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
Cancer Res., 2017-07-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression
Authors: M Garstkiewi, GE Strittmatt, S Grossi, J Sand, G Fenini, S Werner, LE French, HD Beer
Eur. J. Immunol, 2017-04-10;0(0):.
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Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific
Authors: N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi
Front Immunol, 2017-04-03;8(0):379.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity
Authors: E Naschberge, W Gei beta dörfer, C Bogdan, P Tripal, E Kremmer, M Stürzl, N Britzen-La
J. Cell. Mol. Med, 2017-03-08;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
Sci Rep, 2016-12-15;6(0):39035.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals
Authors: R A Steinman
Breast Cancer Res. Treat., 2016-11-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
Authors: Wan-Chien Cheng
Eur J Immunol, 2016-08-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
Arch Med Sci, 2016-07-20;12(5):950-958.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.
Authors: Oliver R, Lovric V, Yu Y, Christou C, Aiken S, Cooper J, Walsh W
PLoS ONE, 2015-08-25;10(8):e0136514.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium.
Authors: Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S
J Biol Chem, 2015-08-12;290(40):24067-78.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-beta Responses.
Authors: Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh V, Chandran B
PLoS Pathog, 2015-07-02;11(7):e1005019.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells.
Authors: Nieminen J, Sipponen T, Farkkila M, Vaarala O
Clin Exp Immunol, 2014-07-01;177(1):190-202.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
PLoS Biol, 2012-09-25;10(9):e1001395.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
Authors: Nicolaidou V, Wong MM, Redpath AN
PLoS ONE, 2012-07-03;7(7):e39871.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature, 2012-04-26;484(7395):514-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
Eur. J. Immunol., 2009-05-01;39(5):1221-30.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity.
Authors: Li J, Yin HL, Yuan J
J. Cell Biol., 2008-04-14;181(2):321-33.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
Nat. Immunol., 2007-08-05;8(9):942-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
Authors: Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP
Am. J. Respir. Cell Mol. Biol., 2006-05-11;35(4):407-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
Authors: Banks WA, Farr SA, La Scola ME
J. Pharmacol. Exp. Ther., 2001-11-01;299(2):536-41.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
Authors: Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
J. Neurochem., 2000-03-01;74(3):1017-25.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Caspase-4 Is Required for Activation of Inflammasomes
Authors: Gabriel Sollberger, Gerhard E. Strittmatter, Magdalena Kistowska, Lars E. French, Hans-Dietmar Beer
The Journal of Immunology
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Herpesvirus-infected Hofbauer cells activate endothelial cells through an IL-1 beta -dependent mechanism
Authors: Paul Hendrix, Zhonghua Tang, Michelle Silasi, Karen E. Racicot, Gil Mor, Vikki M. Abrahams et al.
Placenta
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Macrophage-derived IL-1 beta and TNF-alpha regulate arginine metabolism in neuroblastoma
Authors: Livingstone Fultang, Laura D. Gamble, Luciana Gneo, Andrea M. Berry, Sharon A. Egan, Fenna De Bie et al.
Cancer Research
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Succinate/NLRP3 Inflammasome Induces Synovial Fibroblast Activation: Therapeutical Effects of Clematichinenoside AR on Arthritis.
Authors: Li Y, Zheng JY, Liu JQ et al.
Front Immunol.
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MicroRNA-132 Negatively Regulates Palmitate-Induced NLRP3 Inflammasome Activation through FOXO3 Down-Regulation in THP-1 Cells
Authors: Hye-Eun Byeon, Ja Young Jeon, Hae Jin Kim, Dae Jung Kim, Kwan-Woo Lee, Yup Kang et al.
Nutrients
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A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis
Authors: Alma-Martina Cepika, Romain Banchereau, Elodie Segura, Marina Ohouo, Brandi Cantarel, Kristina Goller et al.
Journal of Experimental Medicine
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Mitochondrial DNA has a pro-inflammatory role in AMD
Authors: Bernard Dib, Haijiang Lin, Daniel E. Maidana, Bo Tian, John B. Miller, Peggy Bouzika et al.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
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Mycobacterial Secretion Systems ESX-1 and ESX-5 Play Distinct Roles in Host Cell Death and Inflammasome Activation
Authors: Abdallah M. Abdallah, Jovanka Bestebroer, Nigel D. L. Savage, Karin de Punder, Maaike van Zon, Louis Wilson et al.
The Journal of Immunology
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PKR deficiency alleviates pulmonary hypertension via inducing inflammasome adaptor ASC inactivation
Authors: Yapei Li, Ying Li, Lijun Li, Minghui Yin, Jiangang Wang, Xiaohui Li
Pulmonary Circulation
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PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation
Authors: Zhouyangfan Peng, Xiqing Tan, Liangpeng Xie, Ze Li, Sufang Zhou, Yapei Li
iScience
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Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells.
Authors: Hubo M et al.
PLoS One
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Average Rating: 4.4 (Based on 8 Reviews)
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We used this IL1B (5ng/mL) neutralizing antibody as part of an antibody cocktail containing several other antibodies to neutralize IL1B present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II. The effect of cytokines in the CM (+/-Neutralizing ab Cocktail) on Endothelial (HUVEC) Cell migration was measured using Incucyte Zoom Chemotaxis Assay.
