Human IL-1 beta /IL-1F2 Antibody Summary
Ala117-Ser269
Accession # NP_000567
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL-1 beta /IL-1F2 in Human Tonsil Using Dual RNAscope®ISH and IHC. IL-1 beta /IL-1F2 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 310361) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).
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Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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IL‑1 beta /IL‑1F2 in Human Tonsil. IL-1 beta /IL-1F2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 39 kDa (as indicated) using 1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 6-42 ng/mL.
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Detection of Mouse IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry Pyroptosis/IL-1 beta pathway is enhanced in vivo and can be modulated by anakinra. a In the PI model, phosphorylated-p65 NF-kappa B, NLRP3, 8-OHdG, IL-1 beta, and TUNEL were detected by IHC (Bar, 100 μm). b IL-1 beta was immuno-localized in gum tissues of mice with PI alone and with orthotopic mammary tumor introduction (Bar, 100 μm). c The mRNA expression of IL-1 beta was measured in the gingiva of the indicated groups. Serum IL-1 beta was detected by ELISA (n ≥ 5; Student’s t test). d The size of primary tumors was measured (n ≥ 7). e Early metastasis in lymph nodes were measured by luciferase assay and normalized by protein concentration, where anakinra reduced the metastasis to the axillary and cervical lymph nodes. MDSC content was also decreased by anakinra in cervical lymph node. (n ≥ 7; Ank anakinra; ANOVA, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31685946), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Immunohistochemistry TMZ inhibits tumor growth and enhances the expression of NLRP1, IL-1 beta, and Notch1 in 1205Lu tumors in vivo. (A) Tumor growth curve of 1205Lu parental cells injected subcutaneously in mice, intraperitoneally treated with a TMZ cycle or 10% dimethyl sulfoxide (DMSO) (Control) (indicated by arrows). Tumor growth was monitored for 26 days. Data are expressed as the mean ± SEM. n = 10 tumors (control) or 12 tumors (TMZ). (B) qRT-PCR analysis of NLRP1 expression in single tumor cells isolated from the tumor tissues studied in (A). Data are expressed as the mean ± SEM. n = 3 samples. * p < 0.05 and *** p < 0.001 vs the corresponding tumors. (C–E) Immunohistochemical staining of tumor tissues with NLRP1 (C), IL-1 beta (D), and Notch1 (E). Samples MB656, MB657, and MB658 were from the control group, whereas MB659, MB660, and MB661 were from the TMZ group. Bar = 50 μm (left panels, 20×) or 20 μm (right panels, 63×). Representative images are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32899791), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Western Blot BTK inhibitors and its dysfunctional mutation suppress NLRP3 inflammasome activation.(a) Enzyme-linked immunosorbent assay (ELISA) of human IL-1 beta in supernatants and immunoblot analysis of human IL-1 beta p17, caspase-1 p20/p22 in supernatants and pro-IL-1 beta in cell lysates of THP-1-Mφs that were pretreated with the indicated inhibitors for 30 min and then stimulated with alum for 6 h. ELISA of murine IL-1 beta (b,c) and TNF-alpha (b) in supernatants of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum for 3 h. (d,e) Immunoblot analysis of the indicated proteins (d) and ELISA of murine IL-1 beta and IL-6 in supernatants of LPS-primed peritoneal macrophages from Xid and WT mice stimulated with alum for 6 h. (f) ELISA of murine IL-1 beta in supernatants of LPS-primed murine peritoneal macrophages pretreated with LFM-A13 and/or Syk inhibitor (R406), then stimulated with alum for 3 h. (g) ELISA of murine IL-1 beta and immunoblot analysis of murine caspase-1 p20 in supernatant of LPS-primed murine peritoneal macrophages stimulated with the indicated NLRP3 inflammasome activators for 3 h. Immunoblot analysis of murine IL-1 beta p17, caspase-1 p20 in supernatants, pro-IL-1 beta, pro-caspase-1 and ASC in cell lysates (h), and ELISA of murine IL-1 beta in supernatants (i) of LPS-primed murine peritoneal macrophages that were pretreated with LFM-A13 and then stimulated with alum or poly(dA:dT) for 3 h. Data are representative of three independent experiments. Data are presented as mean±s.d. (triplicate). **P<0.01; ***P<0.003. Two-sided Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26059659), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 beta/IL-1F2 by Immunohistochemistry IL-1 and IL-1R1 expression is enriched in the melanoma stroma. (A) Real-time qPCR analysis of IL1A and IL1B expression in stage-III and stage-IV melanoma tumor samples (n = 39) relative to expression in human skin samples (n = 8). ***, P < 0.001; Mann-Whitney test. (B) Analysis of IL1B expression in normal skin and benign nevi samples (nonmalignant; n = 25) and cutaneous melanoma samples (malignant; n = 45) from an available gene expression dataset (Talantov et al., 2005) accessed through the Oncomine platform. (C) Sections from a case of primary cutaneous melanoma stained for IL-1 beta, CD163, and CD68 expression as indicated by labels. Bars: (i) 200 µm; (ii) 50 µm; (iii) 33 µm. (D) Serial sections from two skin metastases (i–iii and iv–vi, respectively), stained for IL-1R1 and SMA expression as indicated by the labels. Bars: (i and iv) 200 µm; (ii, iii, v, and vi) 33 µm. (C and D) Arrowheads indicate cells that are clearly double stained. (E) Western blot analysis of IL-1R1 and IL-1 beta precursor protein expression in a panel of cell lines. Data are representative of three independent experiments. (F) Secreted IL-1 beta in conditioned media from a panel of melanoma cell lines detected by ELISA. Data are represented as mean ± SEM for three independent samples in each group. **, P < 0.01; Dunn’s multiple comparisons test. (E and F) Macrophages (Mφ) were stimulated with 100 ng/ml IFN-gamma and 20 ng/ml LPS. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28450382), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Human IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Protein kinase D at the Golgi controls NLRP3 inflammasome activation
Authors: Zhirong Zhang, Gergö Meszaros, Wan-ting He, Yanfang Xu, Helena de Fatima Magliarelli, Laurent Mailly et al.
Journal of Experimental Medicine
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Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores
Authors: Coralie Guy, Marcin Baran, Pau Ribó-Molina, Bernadette G. van den Hoogen, Andrew G. Bowie
iScience
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Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica
Authors: Marrocco A, Frawley K, Pearce LL et al.
The Journal of Immunology
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NLRP3 mutation and cochlear autoinflammation cause syndromic and nonsyndromic hearing loss DFNA34 responsive to anakinra therapy
Authors: Hiroshi Nakanishi, Yoshiyuki Kawashima, Kiyoto Kurima, Jae Jin Chae, Astin M. Ross, Gineth Pinto-Patarroyo et al.
Proceedings of the National Academy of Sciences
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Cadmium-induced ER stress and inflammation are mediated through C/EBP–DDIT3 signaling in human bronchial epithelial cells
Authors: Jeeyoung Kim, Haengseok Song, Hye-Ryeon Heo, Jung Woon Kim, Hye-Ryun Kim, Yoonki Hong et al.
Experimental & Molecular Medicine
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Phenotype and Response to PAMPs of Human Monocyte-Derived Foam Cells Obtained by Long-Term Culture in the Presence of oxLDLs
Authors: Nogiec A, Bzowska M, Demczuk A
Front Immunol
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CARD8 inflammasome activation triggers pyroptosis in human T cells
Authors: Linder A, Bauernfried S, Cheng Y et al.
EMBO J.
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FGF19‐Induced Inflammatory CAF Promoted Neutrophil Extracellular Trap Formation in the Liver Metastasis of Colorectal Cancer
Authors: Chen Li, Tianli Chen, Jialiang Liu, Yue Wang, Chunhuan Zhang, Lu Guo et al.
Advanced Science
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Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production
Authors: Tola A. Faraj, Cordula Stover, Clett Erridge
Frontiers in Immunology
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ABCA1 deficiency contributes to podocyte pyroptosis priming via the APE1/IRF1 axis in diabetic kidney disease
Authors: Ito M, Ducasa GM, Molina JD et al.
Scientific reports
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alpha ‐Synuclein evokes NLRP3 inflammasome‐mediated IL ‐1 beta secretion from primary human microglia
Authors: Adrianne F. Pike, Tatiana Varanita, Maaike A. C. Herrebout, Bonnie C. Plug, Jeroen Kole, René J. P. Musters et al.
Glia
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C4b-binding protein inhibits particulate- and crystalline-induced NLRP3 inflammasome activation
Authors: Damien Bierschenk, Nikolina Papac-Milicevic, Ian P. Bresch, Valentina Kovacic, Serena Bettoni, Mateusz Dziedzic et al.
Frontiers in Immunology
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HIF-1 alpha regulates IL-1 beta and IL-17 in sarcoidosis
Authors: Jaya Talreja, Harvinder Talwar, Christian Bauerfeld, Lawrence I Grossman, Kezhong Zhang, Paul Tranchida et al.
eLife
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Interferon-lambda 3 Exacerbates the Inflammatory Response to Microbial Ligands: Implications for SARS-CoV-2 Pathogenesis
Authors: Scott A Read, Brian S Gloss, Christopher Liddle, Jacob George, Golo Ahlenstiel
Journal of Inflammation Research
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A flagellin-conjugate protein induces dual NLRC4- and NLRP3-inflammasome activation which modulates inflammatory cytokine secretion from macrophages
Authors: Yen-Ju Lin, Annette Jamin, Sonja Wolfheimer, Anna Fiedler, Ann-Christine Junker, Alexandra Goretzki et al.
Frontiers in Immunology
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Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function
Authors: Sinclair, JE;Vedelago, C;Ryan, FJ;Carney, M;Redd, MA;Lynn, MA;Grubor-Bauk, B;Cao, Y;Henders, AK;Chew, KY;Gilroy, D;Greaves, K;Labzin, L;Ziser, L;Ronacher, K;Wallace, LM;Zhang, Y;Macauslane, K;Ellis, DJ;Rao, S;Burr, L;Bain, A;Karawita, A;Schulz, BL;Li, J;Lynn, DJ;Palpant, N;Wuethrich, A;Trau, M;Short, KR;
Nature microbiology
Species: Human
Sample Types: Plasma
Applications: Bioassay -
Vaccinia virus F1L blocks the ribotoxic stress response to subvert ZAK?-dependent NLRP1 inflammasome activation
Authors: Szymanska, I;Bauernfried, S;Komar, T;Hornung, V;
European journal of immunology
Species: Human
Sample Types: Transfected Whole Cells
Applications: Western Blot -
Euphorbia factor L2 alleviated gouty inflammation by specifically suppressing both the priming and activation of NLRP3 inflammasome
Authors: Li, Y;Zhuang, Y;Chen, Y;Wang, G;Tang, Z;Zhong, Y;Zhang, Y;Wu, L;Ji, X;Zhang, Q;Pan, B;Luo, Y;
International immunopharmacology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting IL-1 controls refractory pityriasis rubra pilaris
Authors: Schmauch, E;Severin, Y;Xing, X;Mangold, A;Conrad, C;Johannsen, P;Kahlenberg, JM;Mellett, M;Navarini, A;Nobbe, S;Sarkar, MK;Satyam, A;Tsoi, LC;French, LE;Nilsson, J;Linna-Kuosmanen, S;Kaikkonen, MU;Snijder, B;Kellis, M;Gudjonsson, JE;Tsokos, GC;Contassot, E;Kolios, AGA;
Science advances
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Interleukin-1?/Interleukin (IL)-1-Receptor-Antagonist (IL1-RA) Axis in Invasive Bladder Cancer-An Exploratory Analysis of Clinical and Tumor Biological Significance
Authors: Vukovic, M;Chamlati, JM;Hennenlotter, J;Todenhöfer, T;Lütfrenk, T;Jersinovic, S;Tsaur, I;Stenzl, A;Rausch, S;
International journal of molecular sciences
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Guarea microcarpa C. DC. extract inhibits NLRP3 inflammasome by suppressing its ATPase activity
Authors: Lee, S;Yun, S;Yang, H;Lee, N;Kim, Y;Lee, S;Zamora, NA;Montero, SS;Yi, DK;Kim, SY;Choi, S;Choi, T;Kim, MS;Lee, Y;Park, YH;
Journal of ethnopharmacology
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: Western Blot -
The tetrapeptide sequence of IL-18 and IL-1? regulates their recruitment and activation by inflammatory caspases
Authors: Exconde, PM;Hernandez-Chavez, C;Bourne, CM;Richards, RM;Bray, MB;Lopez, JL;Srivastava, T;Egan, MS;Zhang, J;Yoo, W;Shin, S;Discher, BM;Taabazuing, CY;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot, Bioassay -
HIF1alpha-BNIP3-mediated mitophagy protects against renal fibrosis by decreasing ROS and inhibiting activation of the NLRP3 inflammasome
Authors: J Li, Q Lin, X Shao, S Li, X Zhu, J Wu, S Mou, L Gu, Q Wang, M Zhang, K Zhang, J Lu, Z Ni
Cell Death & Disease, 2023-03-17;14(3):200.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
Nature Immunology, 2022-12-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NLRP3 inflammasome triggers interleukin-37 release from human monocytes
Authors: A Gritsenko, R Díaz-Pino, G Lopez-Cast
European Journal of Immunology, 2022-04-28;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IBD-associated G protein-coupled receptor 65 variant compromises signalling and impairs key functions involved in inflammation
Authors: V Mercier, G Boucher, D Devost, K Bourque, A Alikashani, C Beauchamp, A Bitton, S Foisy, P Goyette, G Charron, TE Hébert, JD Rioux
Cellular Signalling, 2022-02-24;93(0):110294.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
Nature Chemical Biology, 2022-02-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Single cell transcriptomic landscape of diabetic foot ulcers
Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
Nature Communications, 2022-01-10;13(1):181.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
Redox Biology, 2021-12-05;48(0):102205.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Dot Blot -
Endolysosomal ion channel MCOLN2 (Mucolipin-2) promotes prostate cancer progression via IL-1beta/NF-kappaB pathway
Authors: H Yu, M Xie, Z Meng, CY Lo, FL Chan, L Jiang, X Meng, X Yao
British Journal of Cancer, 2021-09-21;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica
Authors: Marrocco A, Frawley K, Pearce LL et al.
The Journal of Immunology
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The Blockade of Tumoral IL1&beta-Mediated Signaling in Normal Colonic Fibroblasts Sensitizes Tumor Cells to Chemotherapy and Prevents Inflammatory CAF Activation
Authors: NG Díaz-Marot, G Garcia-Vic, G Polcaro, M Bañuls, N Albert, A Villanueva, DG Molleví
International Journal of Molecular Sciences, 2021-05-07;22(9):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases
Authors: B Zhou, DW Abbott
Cell Reports, 2021-04-13;35(2):108998.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Glycyrrhizin prevents SARS-CoV-2 S1 and Orf3a induced high mobility group box 1 (HMGB1) release and inhibits viral replication
Authors: P Gowda, S Patrick, SD Joshi, RK Kumawat, E Sen
Cytokine, 2021-03-12;142(0):155496.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8
Authors: Q Gong, K Robinson, C Xu, PT Huynh, KHC Chong, EYJ Tan, J Zhang, ZZ Boo, DET Teo, K Lay, Y Zhang, JSY Lim, WI Goh, G Wright, FL Zhong, B Reversade, B Wu
Nature Communications, 2021-01-08;12(1):188.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inflammasome Sensor NLRP1 Confers Acquired Drug Resistance to Temozolomide in Human Melanoma
Authors: Z Zhai, JM Samson, T Yamauchi, PK Vaddi, Y Matsumoto, CA Dinarello, D Ravindran, M Fujita
Cancers (Basel), 2020-09-04;12(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Platelets Fuel the Inflammasome Activation of Innate Immune Cells
Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
Cell Rep, 2020-05-12;31(6):107615.
Species: Human
Sample Types:
Applications: Blocking/Neutralizing -
Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation
Authors: Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li
Autophagy, 2020-02-16;0(0):1-24.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interleukin 1&beta/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy
Authors: Y Chen, Z Yang, B Deng, D Wu, Y Quan, Z Min
Oncol. Rep., 2020-01-22;43(3):908-918.
Species: Human
Sample Types: Cell Culture Lysates, Whole Tissue
Applications: IHC, Western Blot -
Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells
Authors: R Cheng, S Billet, C Liu, S Haldar, D Choudhury, M Tripathi, M Hav, A Merchant, T Hu, H Huang, H Zhou, NA Bhowmick
Oncogene, 2019-11-04;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysate, Whole Tissue
Applications: IHC, Western Blot -
PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation
Authors: Q Lin, S Li, N Jiang, X Shao, M Zhang, H Jin, Z Zhang, J Shen, Y Zhou, W Zhou, L Gu, R Lu, Z Ni
Redox Biol, 2019-06-11;26(0):101254.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration
Authors: C De Witte, K Demeyere, S De Bruycke, B Taminiau, G Daube, R Ducatelle, E Meyer, F Haesebrouc
Vet. Res., 2019-05-24;50(1):39.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages
Authors: PJ Goddard, J Sanchez-Ga, SL Slater, M Kalyan, D Ruano-Gall, O Marchès, LÁ Fernández, G Frankel, AR Shenoy
Cell Rep, 2019-04-23;27(4):1008-1017.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1? Protein during Virion-Mediated Activation of the AIM2 Inflammasome
Authors: S Botto, J Abraham, N Mizuno, K Pryke, B Gall, I Landais, DN Streblow, KJ Fruh, VR DeFilippis
MBio, 2019-02-12;10(1):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
ROR? controls inflammatory state of human macrophages
Authors: N Nejati Moh, E Bjørkøy Ta, L Ryan, T Espevik, V Boyartchuk
PLoS ONE, 2018-11-28;13(11):e0207374.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
Authors: L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube
Nat Commun, 2018-10-15;9(1):4260.
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ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells
Authors: JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim
Tuberc Respir Dis (Seoul), 2017-07-03;80(3):247-254.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
J. Exp. Med., 2017-04-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii
Authors: K Hendrawan, CM Whittingto, MC Brandley, K Belov, MB Thompson
J. Exp. Zool. B Mol. Dev. Evol, 2017-03-10;0(0):.
Species: Lizard
Sample Types: Tissue Homogenates
Applications: Western Blot -
Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
Autophagy, 2017-01-01;0(0):0.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
Sci Rep, 2016-11-24;6(0):37625.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation
J Immunol, 2016-08-12;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
Authors: Yong Hwan Park
Nat Immunol, 2016-06-06;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage.
Authors: Edye M, Brough D, Allan S
J Biol Chem, 2015-08-31;290(42):25374-81.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
Authors: Kortmann J, Brubaker S, Monack D
J Immunol, 2015-06-24;195(3):815-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
J Immunol, 2015-04-27;194(11):5472-87.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
Nat Med, 2015-02-16;21(3):248-55.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
Eur J Immunol, 2015-01-19;45(4):1092-102.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
Cytokine, 2014-11-21;71(2):223-31.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Complement C5a potentiates uric acid crystal-induced IL-1beta production.
Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
Eur J Immunol, 2014-10-28;44(12):3669-79.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Early mucosal sensing of SIV infection by paneth cells induces IL-1beta production and initiates gut epithelial disruption.
Authors: Hirao L, Grishina I, Bourry O, Hu W, Somrit M, Sankaran-Walters S, Gaulke C, Fenton A, Li J, Crawford R, Chuang F, Tarara R, Marco M, Baumler A, Cheng H, Dandekar S
PLoS Pathog, 2014-08-28;10(8):e1004311.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC -
Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
J Immunol Res, 2014-03-31;2014(0):593562.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
J Lipid Res, 2013-09-03;54(11):2998-3008.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
Nature, 2012-11-11;492(7427):123-7.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
Haematologica, 2011-05-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
J. Immunol., 2011-03-02;186(7):4175-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
Authors: Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD
Cancer Res., 2008-11-15;68(22):9541-50.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
Cytokine, 2008-11-12;44(3):352-60.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-P -
Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
Biol. Reprod., 2007-02-21;76(6):1009-15.
Species: Amphibian
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochem. Photobiol., 2004-09-01;80(2):231-5.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Authors: Sengupta J, Dhawan L, Ghosh D
Cytokine, 2003-12-21;24(6):277-85.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice.
Authors: Beckerman P, Bi-Karchin J, Park AS et al.
Nat. Med.
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The tetrapeptide sequence of IL-1beta regulates its recruitment and activation by inflammatory caspases
Authors: PM Exconde, C Hernandez-, MB Bray, JL Lopez, T Srivastava, MS Egan, J Zhang, S Shin, BM Discher, CY Taabazuing
bioRxiv : the preprint server for biology, 2023-02-16;0(0):.
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Prohibitin 2-mediated mitophagy attenuates renal tubular epithelial cells injury by regulating mitochondrial dysfunction and NLRP3 inflammasome activation
Authors: Yao Xu, Jingjing Wang, Wangjie Xu, Feng Ding, Wei Ding
American Journal of Physiology-Renal Physiology
-
Poly-gamma-glutamic acid from Bacillus subtilis upregulates pro-inflammatory cytokines while inhibiting NLRP3, NLRC4 and AIM2 inflammasome activation
Authors: Huijeong Ahn, Seung Goo Kang, Sung-il Yoon, Pyeung-Hyeun Kim, Doo Kim, Geun-Shik Lee
Cellular & Molecular Immunology
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Brief Report: Connecting Two Pathways Through Ca2+Signaling: NLRP3 Inflammasome Activation Induced by a HypermorphicPLCG2Mutation
Authors: Jae Jin Chae, Yong Hwan Park, Chung Park, Il‐Young Hwang, Patrycja Hoffmann, John H. Kehrl et al.
Arthritis & Rheumatology
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Role of IL-12p40 in cervical carcinoma
Authors: H J M A A Zijlmans, S Punt, G J Fleuren, J B Trimbos, G G Kenter, A Gorter
British Journal of Cancer
-
Inflammasome Assays In Vitro and in Mouse Models
Authors: Haitao Guo, Jenny P.‐Y. Ting
Current Protocols in Immunology
-
IKK beta primes inflammasome formation by recruiting NLRP3 to the trans-Golgi network
Authors: Niklas A. Schmacke, Fionan O’Duill, Moritz M. Gaidt, Inga Szymanska, Julia M. Kamper, Jonathan L. Schmid-Burgk et al.
Immunity
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Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages
Authors: Jeeyoung Kim, Woo Jin Kim, Kwon-Soo Ha, Eun-Taek Han, Won Sun Park, Se-Ran Yang et al.
International Journal of Stem Cells
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The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3
Authors: Moritz M. Gaidt, Thomas S. Ebert, Dhruv Chauhan, Katharina Ramshorn, Francesca Pinci, Sarah Zuber et al.
Cell
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Boron-Based Inhibitors of the NLRP3 Inflammasome
Authors: Alex G. Baldwin, Jack Rivers-Auty, Michael J.D. Daniels, Claire S. White, Carl H. Schwalbe, Tom Schilling et al.
Cell Chemical Biology
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Early IFN-alpha signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19
Authors: Benjamin Krämer, Rainer Knoll, Lorenzo Bonaguro, Michael ToVinh, Jan Raabe, Rosario Astaburuaga-García et al.
Immunity
-
Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro.
Authors: Gritsenko, A, Yu, S Et al.
Front Immunol
-
NAIP-NLRC4-deficient mice are susceptible to shigellosis
Authors: PS Mitchell, JL Roncaioli, EA Turcotte, L Goers, RA Chavez, AY Lee, CF Lesser, I Rauch, RE Vance
Elife, 2020-10-19;9(0):.
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Activated macrophages promote invasion by early colorectal cancer via an interleukin 1 beta ‐serum amyloid A1 axis
Authors: Gota Sudo, Hironori Aoki, Eiichiro Yamamoto, Akira Takasawa, Takeshi Niinuma, Ayano Yoshido et al.
Cancer Science
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USP7 and USP47 deubiquitinases regulate NLRP3 inflammasome activation
Authors: Pablo Palazón‐Riquelme, Jonathan D Worboys, Jack Green, Ana Valera, Fatima Martín‐Sánchez, Carolina Pellegrini et al.
EMBO reports
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Nonsaponin fractions of Korean Red Ginseng extracts prime activation of NLRP3 inflammasome
Authors: Byung-Cheol Han, Huijeong Ahn, Jiseon Lee, Eunsaem Jeon, Sanghoon Seo, Kyoung Hwa Jang et al.
Journal of Ginseng Research
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Epigenetic Regulation during Fetal Femur Development: DNA Methylation Matters
Authors: María C. de Andrés, Emmajayne Kingham, Kei Imagawa, Antonio Gonzalez, Helmtrud I. Roach, David I. Wilson et al.
PLoS ONE
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These Abs recognize both pro-IL1b (p31) and active, cleaved form (p17) in cell lysates and conditioned media of activated human monocyte derived macrophages and THP-1 derived macrophages. To see p17 IL-1b I used 10% tricine gel due to small size of detected protein.
I use antibody at dilution 1:500
