Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody Summary
Ala117-Ser269
Accession # P01584
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with (A) Mouse Anti-Human IL-1 beta /IL-1F2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC201F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Excess Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) inhibits IC201F staining (B). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Human IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Kinin receptor expression during Staphylococcus aureus infection.
Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
Blood, 2006-05-30;108(6):2055-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
FEBS Lett., 2003-03-13;538(1):149-54.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
Authors: Vamvakopoulos J, Green C, Metcalfe S
Eur. J. Immunol., 2002-10-01;32(10):2988-96.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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