Human IL-3R alpha/CD123 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL-3R alpha/CD123 by Western Blot. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-3R alpha/CD123 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF841) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-3R alpha/CD123 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-3R alpha
Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity human IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.
- Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
Product Datasheets
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