Human IL-5 R alpha /CD125 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑5 and Neutralization by Human IL‑5 R alpha /CD125 Antibody. Recombinant Human IL-5 (205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-5 Ra/CD125 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-253-NA). The ND50 is typically 1-3 µg/mL.
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Detection of IL‑5 R alpha /CD125 in HEK293 human embryonic kidney cell line transfected with human IL-5 receptor alpha vs. HEK293 human embryonic kidney cell line transfected with an irrelevant protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human IL-5 receptor alpha (filled histogram) vs HEK293 human embryonic kidney cell line transfected with an irrelevant protein (open histogram) were stained with Goat Anti-Human IL‑5 R alpha /CD125 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-253-NA) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5 R alpha/CD125
Interleukin 5, produced primarily by activated T cells and mast cells, has diverse biological effects on a variety of cell types. Human IL-5 is a potent eosinophil differentiation and activation factor in vivo and in vitro. Additionally, it has also been reported that IL-5 can stimulate the proliferation and/or differentiation of basophils and B cells. The multiple effects of IL-5 are mediated by binding of the cytokine to specific cell surface receptors expressed on target cells. As is the case with many other cytokines, the functional high-affinity receptor for IL-5 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. In the case of IL-5, the beta subunit is shared with the high affinity receptor complexes for IL-3 and GM-CSF. The beta chain does not bind any of the cytokines in question but is indispensable for the cytokine-mediated signaling.
cDNA clones for the alpha chain (IL-5 R alpha ) of both the mouse and human high affinity IL-5 receptor complexes have been isolated. Human and mouse IL-5 R alpha are both members of the hematopoietin receptor superfamily characterized by the presence of the WSXWS, and a four cysteine residue motif in the extracellular domain of the transmembrane protein. In addition to the cDNA clone encoding the full-length transmembrane protein, cDNA clones that arise from alternative splicing and that encode soluble secreted forms of IL-5 R alpha have been isolated from mouse as well as human cells. A naturally-occurring soluble form of the IL-5 R alpha has been detected in biological fluids of autoimmune-prone mice and mice bearing chronic B cell leukemia (BCL1).
A recombinant human IL-5 soluble receptor alpha has been shown to bind the human IL-5 dimer in a 1:1 ratio and acts as a human IL-5 antagonist. This molecule inhibits the proliferation of IL-5-dependent cell lines and blocks human umbilical cord blood eosinophil differentiation.
Product Datasheets
Citations for Human IL-5 R alpha /CD125 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Three lysine residues in the common beta chain of the interleukin-5 receptor are required for Janus kinase (JAK)-dependent receptor ubiquitination, endocytosis, and signaling.
Authors: Lei JT, Mazumdar T, Martinez-Moczygemba M
J. Biol. Chem., 2011-09-30;286(46):40091-103.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
Separate endocytic pathways regulate IL-5 receptor internalization and signaling.
Authors: Lei JT, Martinez-Moczygemba M
J. Leukoc. Biol., 2008-05-29;84(2):499-509.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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