Human Integrin beta 7 Antibody

Catalog #: AF4669 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4669
AF4669-SP

Detection of Integrin beta 7 in Human Lymphocytes by Flow Cytometry.

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Human Integrin beta 7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin beta 7 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta 2, rhIntegrin beta 5, and rhIntegrin beta 6 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
Glu20-His723
Accession # P26010

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Integrin beta 7

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin beta 7 in Human Lymphocytes by Flow Cytometry. Human blood derived lymphocytes were stained with Human Integrin beta 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4669, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20-723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa 501-648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1, and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.

Entrez Gene IDs

3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)

Alternate Names

Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7