Human ITIH4 Antibody

Catalog #: AF8157
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8157
AF8157-SP

Detection of Human ITIH4 by Western Blot.

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All ITIH4 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ITIH4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ITIH4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human ITIH1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ITIH4
Glu29-Leu930 (Ile85Asn, Pro698Thr)
Accession # Q14624

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ITIH4 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITIH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8157) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITIH4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH4

The inter-alpha -trypsin inhibitor heavy chain (ITIH4), also known as IHRP (inter-alpha -trypsin heavy chain-related protein), PK-120 (plasma kallikrein-sensitive glycoprotein 120), or Gp120 (glycoprotein 120), is a secreted, 120 kDa, 930 amino acid (aa), N-glycosylated Type II acute phase protein. It is upregulated in response to trauma such as ischemic stroke or myocardial infarction, and functions as a trypsin inhibitor. It is activated by Kallikrein by cleavage between aa 688-689, creating 100 kDa and 35 kDa portions. Mature human ITIH4 shares 66% aa sequence identity with mouse and rat ITIH4. Human isoforms lacking aa 621-650 (914, 900 and 845 aa forms), aa 727-765 and 851-866 (845 aa) and having 14 aa inserted after aa 727 (914 aa) have been described. Proteolytic cleavage by plasma kallikrein produces a 100 kDa fragment that is further processed to 70 kDa, and 35 kDa fragments. An O-glycosylated form is reported in urine, but not in plasma.

Long Name

Inter-alpha-Trypsin Inhibitor Heavy Chain H4

Entrez Gene IDs

3700 (Human); 16427 (Mouse); 54404 (Rat)

Alternate Names

DKFZp686G21125; gp120; H4P; heavy chain-like, 1; IHRP; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain H4; ITIH4; ITI-HC4; ITIHL1; PK120; PK-120; plasma kallikrein-sensitive glycoprotein 120; PRO1851

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human ITIH4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Proteomics changes after negative pressure wound therapy in diabetic foot ulcers
Authors: Z Jia, L Liu, S Zhang, X Zhao, L Luo, Y Tang, B Shen, M Chen
Molecular Medicine Reports, 2021-10-05;24(6):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot