Home / Jagged 1 / Human Jagged 1 Antibody

Human Jagged 1 Antibody

Catalog #: AF1277
22 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1277
AF1277-SP
Detection of Human Jagged 1 by Western Blot.
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Product Details
Citations (22)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Jagged 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Jagged 1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Jagged 1 antibody by Western Blot. View Larger

Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Jagged 1 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Neutralization Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. View Larger

Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50 is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References
  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

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Citations for Human Jagged 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome
    Authors: Bersini S, Schulte R, Huang L et al.
    eLife
  2. A phase I study of the combination of ro4929097 and cediranib in patients with advanced solid tumours (PJC-004/NCI 8503)
    Authors: S Sahebjam, P L Bedard, V Castonguay, Z Chen, M Reedijk, G Liu et al.
    British Journal of Cancer
  3. Notch signaling in the dynamics of perivascular stem cells and their niches
    Authors: Pierfrancesco Pagella, Laura de Vargas Roditi, Bernd Stadlinger, Andreas E. Moor, Thimios A. Mitsiadis
    Stem Cells Transl Med
  4. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
    Authors: JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia
    Cell Stem Cell, 2019-07-11;0(0):.
  5. Hepatocyte TLR4 triggers inter-hepatocyte Jagged1/Notch signaling to determine NASH-induced fibrosis
    Authors: Junjie Yu, Changyu Zhu, Xiaobo Wang, KyeongJin Kim, Alberto Bartolome, Paola Dongiovanni et al.
    Science Translational Medicine
  6. Vascular endothelial growth factor blockade rapidly elicits alternative proangiogenic pathways in neuroblastoma
    Authors: Nibal Zaghloul, Sonia L. Hernandez, Jae-O Bae, Jianzhong Huang, Jason C Fisher, Alice Lee et al.
    Int J Oncol
  7. Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1
    Authors: Herrera, JL;Komatsu, M;
    Cell reports
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Neutralization
  8. Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation
    Authors: G Wang, B Heijs, S Kostidis, RGJ Rietjens, M Koning, L Yuan, GL Tiemeier, A Mahfouz, SJ Dumas, M Giera, J Kers, SM Chuva de S, CW van den Be, BM van den Be, TJ Rabelink
    Cell Stem Cell, 2022-11-03;29(11):1580-1593.e7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Dysregulated Notch Signaling in the Airway Epithelium of Children with Wheeze
    Authors: T Iosifidis, EN Sutanto, ST Montgomery, P Agudelo-Ro, K Looi, KM Ling, NC Shaw, LW Garratt, J Hillas, KM Martinovic, E Kicic-Star, S Vijayaseka, FJ Lannigan, PJ Rigby, DA Knight, SM Stick, A Kicic
    Journal of personalized medicine, 2021-12-07;11(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: In-Cell Western (ICW)
  10. Jagged Ligands Enhance the Pro-Angiogenic Activity of Multiple Myeloma Cells
    Authors: MT Palano, D Giannandre, N Platonova, G Gaudenzi, M Falleni, D Tosi, E Lesma, V Citro, M Colombo, I Saltarella, R Ria, N Amodio, E Taiana, A Neri, G Vitale, R Chiaramont
    Cancers (Basel), 2020-09-11;12(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation
    Authors: F De Falco, B Del Papa, S Baldoni, R Sabatini, F Falzetti, M Di Ianni, MP Martelli, F Mezzasoma, M Pelullo, P Marconi, P Sportolett, I Screpanti, E Rosati
    Cell Death Dis, 2018-11-26;9(12):1160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cells
    Authors: M Bellon, R Moles, H Chaib-Mezr, J Pancewicz, C Nicot
    J Hematol Oncol, 2018-09-19;11(1):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Evolution of cancer stem-like cells in endocrine-resistant metastatic breast cancers is mediated by stromal microvesicles
    Authors: P Sansone, M Berishaj, VK Rajasekhar, C Ceccarelli, Q Chang, A Strillacci, C Savini, L Shapiro, R Bowman, C Mastroleo, S De Carolis, L Daly, A Benito-Mar, F Perna, N Fabbri, JH Healey, E Spisni, M Cricca, D Lyden, M Bonafé, J Bromberg
    Cancer Res, 2017-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Interleukin-6 Stimulates Defective Angiogenesis.
    Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
    Cancer Res, 2015-06-16;75(15):3098-107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
    Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
    Cancer Res., 2011-11-07;72(1):66-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Interleukin-6 as a therapeutic target in human ovarian cancer.
    Authors: Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR
    Clin. Cancer Res., 2011-07-27;17(18):6083-96.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  17. Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
    Authors: Roesli C, Mumprecht V, Neri D, Detmar M
    FASEB J., 2008-01-07;22(6):1933-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007-10-25;26(1):279-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. A yes-associated protein 1- Notch1 receptor positive feedback loop promotes breast cancer lung metastasis by attenuating the bone morphogenetic protein 4-SMAD family member 1/5 signaling
    Authors: Lin Zhao, Jianjun Lei, Shanzhi Gu, Yujiao Zhang, Xin Jing, Lu Wang et al.
    Carcinogenesis
  20. Hippo signaling interactions with Wnt/ beta -catenin and Notch signaling repress liver tumorigenesis
    Authors: Wantae Kim, Sanjoy Kumar Khan, Jelena Gvozdenovic-Jeremic, Youngeun Kim, Jason Dahlman, Hanjun Kim et al.
    Journal of Clinical Investigation
  21. A phase Ib combination study of RO4929097, a gamma-secretase inhibitor, and temsirolimus in patients with advanced solid tumors
    Authors: Ivan Diaz-Padilla, Hal Hirte, Amit M. Oza, Blaise A. Clarke, Brenda Cohen, Michael Reedjik et al.
    Investigational New Drugs
  22. The orphan drug dichloroacetate reduces amyloid beta-peptide production whilst promoting non-amyloidogenic proteolysis of the amyloid precursor protein
    Authors: ET Parkin, JE Hammond, L Owens, MD Hodges
    PLoS ONE, 2022-01-13;17(1):e0255715.

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Human Jagged 1 Antibody
By Anonymous on 12/10/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: RT4-D6P2T rat schwannoma cell line Species: Human
Immunocytochemistry/Immunofluorescence Human Jagged 1 Antibody AF1277