Human Kallikrein 3/PSA Antibody

Catalog #: MAB13442
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13442-SP
MAB13442-500
MAB13442-100

Detection of Human Kallikrein 3/PSA by Western Blot.

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Citations (1)

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Human Kallikrein 3/PSA Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 3/PSA in sandwich immunoassays and in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 181811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Pro19-Pro261
Accession # P07288

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Human Kallikrein 3/PSA Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Kallikrein 3/PSA Biotinylated Antibody (Catalog # BAF1344)

Standard: Recombinant Human Kallikrein 3/PSA Protein, CF (Catalog # 1344-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Kallikrein 3/PSA by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kallikrein 3/PSA at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 3/PSA

Kallikrein 3 (KLK-3) is a member of the Kallikrein family of serine proteases. It is also known as PSA (prostate specific antigen) and is used in prostate cancer diagnosis.

Entrez Gene IDs

354 (Human)

Alternate Names

APS seminin; EC 3.4.21; EC 3.4.21.77; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Kallikrein 3/PSA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1