Human Kallikrein 3/PSA Antibody

Catalog #: MAB13441
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13441
MAB13441-SP

Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines.

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Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 3/PSA Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 3/PSA in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Factor X, rhHGF-A, rhKLK-4, -5, -8, -11, or rhThrombin is observed.

Source

Monoclonal Mouse IgG_2B Clone # 181827

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 3/PSA
Pro19-Pro261
Accession # NP_001639

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 3/PSA (10 µg/mL, Catalog # 1344-SE) cleavage of the fluorogenic peptide substrate Suc-RPY-pNA (1 mM). The Neutralization Dose (ND₅₀) is typically 21 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines. Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13441) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Kallikrein 3/PSA in Human Prostate. Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 3/PSA Monoclonal Antibody (Catalog # MAB13441) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 3/PSA

Kallikrein 3 is a member of the Kallikrein family of serine proteases. It is also known as PSA (prostate-specific antigen) and is used in prostate cancer diagnosis.

Entrez Gene IDs

354 (Human)

Alternate Names

APS seminin; EC 3.4.21; EC 3.4.21.77; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Kallikrein 3/PSA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Transient induction of ING4 by Myc drives prostate epithelial cell differentiation and its disruption drives prostate tumorigenesis.
Authors: Berger P, Frank S, Schulz V, Nollet E, Edick M, Holly B, Chang T, Hostetter G, Kim S, Miranti C
Cancer Res, 2014-04-24;74(12):3357-68.
Species: Human
Sample Types: Whole Cells
Applications: IHC