Human Kallikrein 9 Antibody

Catalog #: AF3486
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3486
AF3486-SP

Kallikrein 9 in Human Ovarian Cancer Tissue.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human KLK-1, -3, -4, -5, -6, -7, -8, -10, -11, -12, -13, -14, and -15 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 9
Asp20-Asn250
Accession # Q9UKQ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Kallikrein 9

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kallikrein 9, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Kallikrein 9 in Human Ovarian Cancer Tissue. Kallikrein 9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3486) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 9

The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.

Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.

Entrez Gene IDs

284366 (Human); 101533 (Mouse); 292868 (Rat)

Alternate Names

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Kallikrein 9; Kallikrein-like protein 3; kallikrein-related peptidase 9; KLK9; KLKL3; KLK-L3; KLK-L3kallikrein-9; MGC50513

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Kallikrein 9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
Nat. Biotechnol., 2020-02-24;0(0):.
Species: Human
Sample Types: Cells
Applications: ICC
HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
Nat. Immunol., 2020-02-17;21(3):274-286.
Species: Human
Sample Types: Cells
Applications: ICC
Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
Authors: Chee J, Singh J, Naran A, Misso NL, Thompson PJ, Bhoola KD
Biol. Chem., 2007-11-01;388(11):1235-42.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P