Human KLF17 Antibody

Catalog #: AF6845 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6845
AF6845-SP

Detection of Human KLF17 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLF17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human KLF17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) KLF1, rhKLF2, rhKLF4, rhKLF5, rhKLF6, rhKLF10, and rhKLF12 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human KLF17
Met1-Gln228
Accession # Q5JT82

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human KLF17 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6845) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF17 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF17

KLF17 (Kruppel-like factor 17) is a 38-39 kDa member of the Sp1 C2H2-type zinc-finger protein family. Based on mouse studies, it is expressed in germ cells and likely plays a role in spermatid and oocyte differentiation. It is also found to be downregulated in tumors where, if present, it normally suppresses Id1 (Inhibitor of DNA binding 1), a transcription factor associated with tumorigenesis. Human KLF17 is 389 amino acids (aa) in length and contains three zinc-finger motifs (aa 283‑365). There is one potential alternate splice form that shows a 19 aa substitution for aa 97-389. Over aa 1-228, human KLF17 shares only 33% aa identity with mouse ZFP393/KLF17.

Long Name

Kruppel-Like Factor 17

Entrez Gene IDs

128209 (Human); 75753 (Mouse); 298449 (Rat)

Alternate Names

FLJ40160; KLF17; Kruppel-like factor 17; Zfp393; Zinc finger protein 393Krueppel-like factor 17; ZNF393; ZNF393novel zinc-finger protein