Human LILRA4/CD85g/ILT7 Antibody

Catalog #: MAB62871 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB62871-100
MAB62871-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRA4/CD85g/ILT7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA4/CD85g/ILT7 in ELISA. No cross-reactivity with human LILRA1, LILRA2, LILRA3, LILRA5, LILRA6, LILRB2 and LILRB3 with ELISA was observed.

Source

Monoclonal Mouse IgG_2A Clone # 656656

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0derived human LILRA4/CD85g/ILT7
Glu24-Asn446
Accession # P59901

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

In a functional ELISA binding assay, 0.1-1.2 ug/mL of this antibody will block 50% of the binding of 0.3 ug/mL of Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (Catalog # 914-AN) when Recombinant Human LILRA4/CD85g/ILT7 (Catalog # 8914-T4) is immobilized at 1 ug/mL (100 µL/well). At 10 ug/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA4/CD85g/ILT7

LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Alternative splicing generates an additional isoform that lacks the signal peptide and a portion of the first Ig-like domain. LILRA4 is expressed on plasmacytoid dendritic cells (pDC) but is down-regulated in response to TLR9 signaling (3-5). Antibody mediated crosslinking of LILRA4 on pDC inhibits the production of type I interferons following TLR7 or TLR9 stimulation (3, 4, 6). It also blocks the up-regulation of CCR7 but enhances the up-regulation of Integrin beta 7 on TLR7/9-stimulated pDC (6). LILRA4 associates with the ITAM-containing adaptor protein Fc epsilon RI gamma (3, 4, 6), and this complex binds to cell surface BST2/Tetherin which is expressed on monocytes, plasmacytoid and myeloid dendritic cells, B cells, and activated CD4⁺ and CD8⁺ T cells (5, 8). This interaction inhibits the TLR-induced pDC production of type I interferons, IL-6, and TNF-alpha (8).

References

Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
Young, N.T. et al. (2001) Immunogenetics 53:270.
Cho, M. et al. (2008) Int. Immunol. 20:155.
Cao, W. et al. (2006) J. Exp. Med. 203:1399.
Tavano, B. et al. (2013) J. Immunol. 190:2622.
Tsukamoto, N. et al. (2009) Clin. Cancer Res. 15:5733.
Tavano, B. and A. Boasso (2014) PLoS ONE 9:e89414.
Cao, W. et al. (2009) J. Exp. Med. 206:1603.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4

Entrez Gene IDs

23547 (Human)

Alternate Names

CD85 antigen-like family member G; CD85g antigen; CD85g; ILT7; ILT-7; ILT7MGC129598; Immunoglobulin-like transcript 7; leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member4; LILRA4; MGC129597