Human LILRB2/CD85d/ILT4 Biotinylated Antibody

Catalog #: BAF2078
2 Citations Datasheet / COA / SDS

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BAF2078

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB2/CD85d/ILT4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB2/CD85d/ILT4 in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) ILT2 is observed and less than 5% cross-reactivity with rhILT5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB2/CD85d/ILT4
Gly24-His458
Accession # AAC51882

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4)

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB2/CD85d/ILT4

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1‑3). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC) (4). Human ILT4 is produced as a 598 amino acid (aa) precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain. The ECD contains four Ig-like domains, and the cytoplasmic domain contains three immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64% aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5‑9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1 (4, 6). Activation of ILT4 inhibits signaling through Fc gamma RI (4) and Fc epsilon RI (6) and causes DC to become tolerogenic by down-regulation of
co-stimulatory molecules (10, 11). ILT4 mediates tolerogenic DC-induced CD4⁺ T cell energy in vitro and in vivo (10‑12).

References

Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7.
Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317.
Hunt, J.S. et al. (2005) FASEB J. 19:681.
Finger, N.A. et al. (1998) Eur. J. Immunol. 28:3423.
Borges, L. et al. (1997) J. Immunol. 159:5192.
Colonna, M. et al. (1998) J. Immunol. 160:3096.
Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. 100:8856.
Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
Allen, R.L. et al. (2001) J. Immunol. 167:5543.
Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133.
Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5

Entrez Gene IDs

10288 (Human)

Alternate Names

CD85 antigen-like family member D; CD85d antigen; CD85d; Ig-like transcript 4; ILT4; ILT-4; ILT4CD85d; Immunoglobulin-like transcript 4; Leukocyte immunoglobulin-like receptor 2; leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB2; LIR2; LIR2CD85D; LIR-2subfamily A (with TM domain), member 6; member 2; MIR10; MIR10LILRA6; Monocyte/macrophage immunoglobulin-like receptor 10

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Citations for Human LILRB2/CD85d/ILT4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression.
Authors: Banerjee S, Lin CF, Skinner KA, Schiffhauer LM, Peacock J, Hicks DG, Redmond EM, Morrow D, Huston A, Shayne M, Langstein HN, Miller-Graziano CL, Strickland J, O'Donoghue L, De AK
Cancer Res., 2011-01-11;71(2):318-27.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Cytokine induced expression of programmed death ligands in human neutrophils.
Authors: Bankey PE, Banerjee S, Zucchiatti A, De M, Sleem RW, Lin CF, Miller-Graziano CL, De AK
Immunol. Lett., 2010-02-01;129(2):100-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry