Human LRMP Antibody

Catalog #: AF6869 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6869
AF6869-SP

Detection of Human LRMP by Western Blot.

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Human LRMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRMP in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LRMP
Gln143-Pro292
Accession # AAI26418

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LRMP by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRMP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRMP

LRMP (Lymphoid restricted membrane protein; also JAW1) is a 74-76 kDa protein that is associated with the ER. In contrast to its name, LRMP expression is not restricted to lymphoid cell types. Cells known to express LRMP include stomach chief cells, seminal vesicle cuboidal epithelium, neurons, germinal center B cells, cortical thymocytes, and cells of the taste buds. LRMP is suggested to participate in intracellular peptide transport. It is also reported to physically interact with IP3RP. Human LRMP is a 56 kDa (predicted), single-pass transmembrane (TM) protein that is 499 amino acids (aa) in length. It contains a 439 aa cytoplasmic region (aa 1-439), a 21 aa TM segment (aa 440-460) and a 39 aa luminal domain (aa 461-499) that is cleaved after Ala469 to generate a type IV TM protein. LRMP runs anomalously in SDS-Page, and the 75 kDa form may be linked to an isoform that utilizes a Leu start site 56 aa upstream of the start site for the 499 aa isoform. In this regard, a human isoform has been reported that is 62 kDa in size. Mature mouse LRMP is 509 aa in length (after cleavage) and runs at 65 kDa in SDS-PAGE. Over aa 143-292, human LRMP shares 93% aa identity with mouse LRMP.

Long Name

Lymphoid-restricted Membrane Protein

Entrez Gene IDs

4033 (Human); 16970 (Mouse); 500361 (Rat)

Alternate Names

Jaw1; JAW1Protein Jaw1; LRMP; lymphoid-restricted membrane protein