Home / LRRC32/GARP / Human LRRC32/GARP DuoSet ELISA

Human LRRC32/GARP DuoSet ELISA

Catalog #: DY6055-05
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY6055-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human LRRC32 / GARP ELISA Standard Curve
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human LRRC32/GARP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human LRRC32 / GARP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human LRRC32 / GARP ELISA Standard Curve View Larger

Human LRRC32 / GARP ELISA Standard Curve

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LRRC32/GARP

TGF-beta binding proteins 1-4/Latent TGF-beta binding proteins 1-4 (LTBPs 1-4) are members of the LTBP/fibrillin family of proteins. They are characterized by the presence of multiple EGF-like repeats and a series of cysteine-rich repeats. Extracellularly, LTBPs form part of a large inactive TGF-beta complex where they serve to anchor a mature, dimeric TGF-beta/TGF-beta propeptide complex to the ECM.

Long Name:
Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
Entrez Gene IDs:
2615 (Human); 434215 (Mouse)
Alternate Names:
D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32

Citation for Human LRRC32/GARP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GARP Regulates the Immune Capacity of a Human Autologous Platelet Concentrate
    Authors: ER Trzeciak, N Zimmer, PW Kämmerer, D Thiem, B Al-Nawas, A Tuettenber, S Blatt
    Biomedicines, 2022-12-05;10(12):.
    Species: Human
    Sample Types: Cell Lysates

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Loading...

Reviews for Human LRRC32/GARP DuoSet ELISA

There are currently no reviews for this product. Be the first to review Human LRRC32/GARP DuoSet ELISA and earn rewards!

Have you used Human LRRC32/GARP DuoSet ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review