Human Ly6K Antibody

Catalog #: MAB66481 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB66481-100
MAB66481-SP

Detection of Ly6K in HeLa (positive) and K562 (negative) cells.

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Human Ly6K Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ly6K in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1053415

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
Met1-Gly138
Accession # Q17RY6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Human Testes

Immunocytochemistry

8-25 µg/mL

Immersion fixed HeLa (positive) and K562 (negative) cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Ly6K in HeLa (positive) and K562 (negative) cells. Ly6K was detected in immersion fixed HeLa (positive) and K562 (negative) cells using Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of Human Ly6K by Western Blot. Western blot shows lysates of human Testes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ly6K at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly6K

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Long Name

Lymphocyte Antigen 6 Complex Locus K

Entrez Gene IDs

54742 (Human); 76486 (Mouse)

Alternate Names

cancer/testis antigen 97; CO16; CT97; FLJ35226; HSJ001348; Ly6K; ly-6K; lymphocyte antigen 6 complex, locus K; lymphocyte antigen 6K