Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody

Catalog #: AF6746 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6746
AF6746-SP

Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A by Western Blot.

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All Lysine (K)-specific Demethylase 3A/KDM3A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lysine (K)-specific Demethylase 3A/KDM3A Antibody Summary

Species Reactivity

Human

Specificity

Detects human KDM3A in direct ELISAs and Western blots. In direct ELISAs, approximately 3% cross-reactivity with recombinant human (rh) KDM4A is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human KDM3A
Pro844-Glu968
Accession # Q9Y4C1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Lysine (K)-specific Demethylase 3A/KDM3A by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human KDM3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6746) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KDM3A at approximately 150 kDa (as indicated), the band at approximately 70kDa represents the product of degradation or processing. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysine (K)-specific Demethylase 3A/KDM3A

JMJD1A (Jumonji [Japanese for "cruciform/cross"]/ARID domain-containing protein 1A; also KDM3A, JHDM2A, and TSGA) is a 140-150 kDa member of the JHDM2 histone demethylase family of proteins. It is induced by hypoxia, and expressed in spermatids, smooth muscle cells, mammary epithelium, hepatocytes, and renal tubular epithelium. JMJD1A is reported to demethylate di- and mono-methylated Lys9 on histone H3. It has no activity on trimethylated Lys9. JMJD1A is both cytoplasmuc and nuclear, and serves as a coactivator for the AR. Human JMJD1A is 1321 amino acids (aa) in length. It contains one C6-type zinc-finger motif (aa 662-687) and a jumonji C type catalytic domain (aa 1151-1264). There is one phosphorylation site at Ser445. There is one potential splice variants that shows a 13 aa substitution for aa 699-1321. Over aa 810-968, human JMJD1A shares 94% aa identity with mouse JMJD1A.

Entrez Gene IDs

55818 (Human); 104263 (Mouse); 312440 (Rat)

Alternate Names

EC 1.14.11; EC 1.14.11.-; JHDM2A; JHDM2AJMJD1AJHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; jumonji C domain-containing histone demethylase 2A; jumonji domain containing 1; jumonji domain containing 1A; Jumonji domain-containing protein 1A; KDM3A; KIAA0742; KIAA0742DKFZp686A24246; Lysine (K)specific Demethylase 3A; Lysine (K)-specific Demethylase 3A; lysine-specific demethylase 3A; testis-specific protein A; TSGA; TSGADKFZp686P07111