Human MCAM/CD146 PE-conjugated Antibody

Catalog #: FAB932P
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB932P-100
FAB932P-025

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry.

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All MCAM/CD146 Antibodies

Product Details

Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCAM/CD146 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1.

Source

Monoclonal Mouse IgG₁ Clone # 128018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCAM
Val24-Gly559
Accession # AAA20824

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 PE-conjugated Monoclonal Antibody (Catalog # FAB932P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name

Melanoma Cell Adhesion Molecule

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Alternate Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human MCAM/CD146 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Repertoire of endothelial progenitor cells mobilized by femoral artery ligation: a nonhuman primate study
Authors: Qiang Shi, Laura A. Cox, Vida Hodara, Xing Li Wang, John L. VandeBerg
Journal of Cellular and Molecular Medicine
Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro*
Authors: Letícia Martins SANTOS, Patricia e Silva CARDOSO, Elisa Abreu DINIZ, Juliana Garuba RAHHAL, Carla Renata SIPERT
Journal of Applied Oral Science
Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections
Authors: Anne-Claire Lagrée, Fabienne Fasani, Clotilde Rouxel, Marine Pivet, Marie Pourcelot, Aurore Fablet et al.
International Journal of Molecular Sciences
Impaired repair properties of endothelial colony-forming cells in patients with granulomatosis with polyangiitis
Authors: APT Del Rio, JO Frade-Guan, S Ospina-Pri, BKL Duarte, MB Bertolo, MC Ozelo, Z Sachetto
Journal of Cellular and Molecular Medicine, 2022-09-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
PLoS ONE, 2014-01-29;9(1):e84417.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
Stem Cells Dev, 2013-12-04;23(5):541-54.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
EBNA3B-deficient EBV promotes B cell lymphomagenesis in humanized mice and is found in human tumors.
J. Clin. Invest., 2012-03-12;122(4):1487-502.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
Authors: Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
Cytometry A, 2011-07-06;79(8):635-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry