Human MDL-1/CLEC5A Antibody

Catalog #: MAB2384
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2384
MAB2384-SP

Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry.

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All MDL-1/CLEC5A Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MDL-1/CLEC5A Antibody Summary

Species Reactivity

Human

Specificity

Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.

Source

Monoclonal Mouse IgG_2B Clone # 283834

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
Tyr26-Lys188
Accession # Q9NY25

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MDL‑1/CLEC5A under non-reducing conditions only

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human MDL-1/CLEC5A Monoclonal Antibody (Catalog # MAB2384, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.

Long Name

Myeloid DAP12-associating Lectin

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Alternate Names

CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MDL-1/CLEC5A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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MAFB Determines Human Macrophage Anti-Inflammatory Polarization: Relevance for the Pathogenic Mechanisms Operating in Multicentric Carpotarsal Osteolysis
Authors: Víctor D. Cuevas, Laura Anta, Rafael Samaniego, Emmanuel Orta-Zavalza, Juan Vladimir de la Rosa, Geneviève Baujat et al.
The Journal of Immunology
Cutting Edge: CLEC5A Mediates Macrophage Function and Chronic Obstructive Pulmonary Disease Pathologies
Authors: BW Wortham, BL Eppert, JL Flury, SM Garcia, WR Donica, A Osterburg, B Joyce-Shai, DJ Cua, MT Borchers
J. Immunol., 2016-02-29;196(8):3227-31.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Atypical Activin A and IL-10 Production Impairs Human CD16+ Monocyte Differentiation into Anti-Inflammatory Macrophages.
Authors: Gonzalez-Dominguez E, Dominguez-Soto A, Nieto C, Flores-Sevilla J, Pacheco-Blanco M, Campos-Pena V, Meraz-Rios M, Vega M, Corbi A, Sanchez-Torres C
J Immunol, 2016-01-04;196(3):1327-37.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Comparison of gene expression profiles between human and mouse monocyte subsets.
Authors: Ingersoll MA, Spanbroek R, Lottaz C
Blood, 2009-11-12;115(3):e10-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry