Human Mesothelin Antibody

Name: Human Mesothelin Antibody
Brand: R&D Systems
SKU: MAB32654
Rating: 4 (1 reviews)

Catalog #: MAB32654 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB32654-100
MAB32654-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mesothelin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mesothelin in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 618922

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Asp580
Accession # AAH09272

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32653).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Mesothelin DuoSet ELISA Kit (Catalog # DY3265) for convenient development of a sandwich ELISA or the Human Mesothelin Quantikine ELISA Kit (Catalog # DMSLN0) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Mesothelin ELISA Standard Curve. Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32653) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Alternate splicing generates additional Mesothelin isoforms that have either an eight amino acid insertion following Ser408 or a substituted C-terminal region with no GPI anchor (6). Within aa 29-580, human Mesothelin shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 7). It is upregulated in mesotheliomas and a range of carcinomas and adenomas (8-11). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (10, 12). It is coexpressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (11, 14, 15).

References

Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
Ordonez, N.G. (2003) Mod. Pathol. 16:192.
Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
Robinson, B.W.S. et al. (2003) Lancet 362:1612.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein