Human Mesothelin Antibody

Catalog #: MAB11417 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11417-100
MAB11417-SP

Detection of Mesothelin in Capan-2 (positive) and K562 cells (negative).

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All Mesothelin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mesothelin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mesothelin in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 618924

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human Mesothelin
Glu296-Gly580
Accession # Q13421

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed Capan‑2 human pancreatic adenocarcinoma cells (positive), and K562 human chronic myelogenous leukemia cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Mesothelin in Capan-2 (positive) and K562 cells (negative). Mesothelin was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cells (positive), and absent in K562 human chronic myelogenous leukemia cells (negative) using Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB11417) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Alternate splicing generates additional Mesothelin isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor (6). This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 7). It is up‑regulated in mesotheliomas and a range of carcinomas and adenomas (8 ‑ 11). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (10, 12). It is coexpressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (11, 14, 15).

References

Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
Ordonez, N.G. (2003) Mod. Pathol. 16:192.
Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
Robinson, B.W.S. et al. (2003) Lancet 362:1612.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein