Human Mesothelin Biotinylated Antibody

Catalog #: BAF3265 Datasheet / COA / SDS

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BAF3265

All Mesothelin Antibodies

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Human Mesothelin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mesothelin in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 1
Glu296-Gly580
Accession # ABW03459

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Mesothelin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic proregion that is removed when a GPI-anchor is added. The N-terminal prosegment is cleaved by furin to release a 33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). The membrane bound portion of Mesothelin can also be shed and is detectable in sera. Within the region used as the immunogen, human Mesothelin shares approximately 58% amino acid sequence identity with the mouse or rat protein.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein