Human Mesothelin Propeptide/MPF Antibody

Catalog #: MAB3265 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3265
MAB3265-SP

All Mesothelin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mesothelin Propeptide/MPF Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mesothelin Propeptide/MPF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Mesothelin is observed.

Source

Monoclonal Mouse IgG₁ Clone # 346633

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin propeptide
Arg35-Leu289
Accession # AAH09272

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Mesothelin Propeptide/MPF

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Megakaryocyte Potentiating Factor (MPF) is a 33 kDa soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% amino acid identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein