Human Mesothelin Propeptide/MPF Antibody

Catalog #: AF3448 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3448
AF3448-SP

All Mesothelin Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mesothelin Propeptide/MPF Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mesothelin Propeptide/MPF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with mature recombinant human Mesothelin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin Propeptide/MPF isoform 1
Arg35-Leu289
Accession # Q13421

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Mesothelin Propeptide/MPF

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin is a 40 kDa, 292 amino acid, GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic pro-region that is removed when a GPI-anchor is added. The amino‑terminal propeptide is cleaved by furin to release a33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). Mouse MPF has been shown to potentiate megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. The amino acid sequence of human MPF is 55% identical to that of mouse or rat MPF.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein