Human Methionine Aminopeptidase 2/
METAP2 Antibody

Catalog #: AF3795
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3795
AF3795-SP

Detection of Human Methionine Aminopeptidase 2 by Western Blot.

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All Methionine Aminopeptidase 2/METAP2 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Methionine Aminopeptidase 2/
METAP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Methionine Aminopeptidase 2/METAP2 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase 2/METAP2
Ala2-Tyr478
Accession # P50579

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 2/METAP2 (Catalog # 3795-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Methionine Aminopeptidase 2 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Methionine Aminopeptidase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Methionine Aminopeptidase 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 2/METAP2

The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.

References

Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.

Entrez Gene IDs

10988 (Human); 56307 (Mouse); 64370 (Rat)

Alternate Names

Amp2; eIF-2-associated p67 homolog; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; METAP2; Methionine Aminopeptidase 2; methionyl aminopeptidase 2; MNPEP; p67; p67EC 3.4.11.18; p67eIF2; Peptidase M 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Methionine Aminopeptidase 2/
METAP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation
Authors: EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin
Cell Reports, 2023-01-16;0(0):111965.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
Nature Chemical Biology, 2022-02-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Pig epiblast stem cells depend on activin/nodal signaling for pluripotency and self-renewal.
Authors: Alberio R, Croxall N, Allegrucci C
Stem Cells Dev., 2010-10-01;19(10):1627-36.
Species: Porcine
Sample Types: Whole Cells
Applications: ICC