Human MMP-2 PE-conjugated Antibody

Catalog #: IC9023P
3 Citations Datasheet / COA / SDS

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IC9023P

Detection of MMP‑2 in MG‑63 Human Cell Line by Flow Cytometry.

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All MMP-2 Antibodies

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Citations (3)

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Human MMP-2 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects the pro and active forms of human MMP-2 in Western blots. In dot blots, no cross-reactivity with recombinant human MMP-1, -3, -7,
-8, -9, -10, -12, or -13 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 1A10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-2
Accession # P08253

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MMP‑2 antibody in MG‑63 Human Cell Line antibody by Flow Cytometry.

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Detection of MMP‑2 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human MMP-2 PE-conjugated Monoclonal Antibody (Catalog # IC9023P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases that have a combined ability to degrade all the components of the extracellular matrix. MMP‑2 (Gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagen, as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP‑1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP‑2 has been shown to be associated with many connective tissue cells, as well as neutrophils, macrophages and monocytes. Structurally, MMP‑2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Alternate Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

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Citations for Human MMP-2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Plasmatic MMP9 released from tumor-infiltrating neutrophils is predictive for bevacizumab efficacy in glioblastoma patients: an AVAglio ancillary study
Authors: C Jiguet-Jig, S Boissonnea, E Denicolai, V Hein, R Lasseur, J Garcia, S Romain, R Appay, T Graillon, W Mason, AF Carpentier, AA Brandes, L' Ouafik, W Wick, A Baaziz, JP Gigan, RJ Argüello, D Figarella-, O Chinot, E Tabouret
Acta neuropathologica communications, 2022-01-03;10(1):1.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Role of matrix metalloproteinases 2 and 9, toll-like receptor 4 and platelet-leukocyte aggregate formation in sepsis-associated thrombocytopenia
Authors: CM Larkin, NK Hante, EP Breen, KA Tomaszewsk, S Eisele, MW Radomski, TA Ryan, MJ Santos-Mar
PLoS ONE, 2018-05-07;13(5):e0196478.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells
Authors: C Lavini-Ram, HM Silva, A Soares-Sch, SM Monteiro, LR Ferreira, AP Pacanaro, S Gomes, J Batista, K Faé, J Kalil, V Coelho
Sci Rep, 2017-04-13;7(1):874.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry