Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody

Catalog #: BAF310
17 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF310

All CXCL12/SDF-1 Antibodies

Product Details

Citations (17)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse CXCL12/SDF-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant human (rh) SDF-1 beta is observed and less than 0.1% cross-reactivity with rhIP‑10 and recombinant mouse CRG‑2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CXCL12/SDF‑1 alpha (R&D Systems, Catalog # 350-NS)
Lys22-Lys89
Accession # Q6ICW0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)

Recombinant Human/Feline CXCL12/SDF-1 beta (Catalog # 351-FS)

Recombinant Mouse CXCL12/SDF-1 alpha (Catalog # 460-SD)

Human/Mouse CXCL12/SDF-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human/Mouse CXCL12/SDF‑1 Antibody (Catalog # MAB350)

Standard: Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS)

Standard: Recombinant Mouse CXCL12/SDF-1 alpha Protein (Catalog # 460-SD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL12/SDF-1

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1 alpha and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

References

Tashiro, K. et al. (1993) Science 261:600.
Bleul, C. et al. (1996) Nature 382:829.
Oberlin, E. et al. (1996) Nature 382:833.

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)

Alternate Names

chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse CXCL12/SDF‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation
Authors: Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al.
Biology of Blood and Marrow Transplantation
Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis
Authors: C Fielding, A García-Gar, C Korn, S Gadomski, Z Fang, JL Reguera, JA Pérez-Simó, B Göttgens, S Méndez-Fer
Nature Communications, 2022-01-27;13(1):543.
Species: Mouse
Sample Types: Bone Marrow Lavage Fluid
Applications: ELISA Detection
Plasma cell dynamics in the bone marrow niche
Authors: Z Benet, Z Jing, DR Fooksman
Cell Reports, 2021-02-09;34(6):108733.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Capture
Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells
Authors: A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia
Immunobiology, 2017-11-22;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
PLoS ONE, 2017-04-05;12(4):e0174447.
Species: Rat
Sample Types: Plasma
Applications: ELISA Development (Detection)
Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
EMBO J, 2017-03-02;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Cholinergic Signals from the CNS Regulate G-CSF-Mediated HSC Mobilization from Bone Marrow via a Glucocorticoid Signaling Relay
Authors: H Pierce, D Zhang, C Magnon, D Lucas, JR Christin, M Huggins, GJ Schwartz, PS Frenette
Cell Stem Cell, 2017-02-09;0(0):.
Species: Mouse
Sample Types: Bone Marrow Fluid
Applications: ELISA Development (Detection)
CXCL12 chemokine expression suppresses human pancreatic cancer growth and metastasis.
Authors: Roy, Ishan, Zimmerman, Noah P, Mackinnon, A Craig, Tsai, Susan, Evans, Douglas, Dwinell, Michael
PLoS ONE, 2014-03-04;9(3):e90400.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Colonic patch and colonic SILT development are independent and differentially regulated events.
Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
Mucosal Immunol, 2012-09-19;6(3):511-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
Cancer Res., 2008-03-15;68(6):1889-95.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development

Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
J. Cell. Physiol., 2006-05-01;207(2):364-73.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development

Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr

Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo.
Authors: Villalba S, Salvucci O, Aoki Y, De La Luz Sierra M, Gupta G, Davis D, Wyvill K, Little R, Yarchoan R, Tosato G
J. Leukoc. Biol., 2003-07-22;74(5):880-8.
Species: Human
Sample Types: Serum
Applications: ELISA Development, Western Blot

Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche
Authors: Andrew Chow, Daniel Lucas, Andrés Hidalgo, Simón Méndez-Ferrer, Daigo Hashimoto, Christoph Scheiermann et al.
Journal of Experimental Medicine

Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells
Authors: Pratibha Singh, Jonathan Hoggatt, Malgorzata M. Kamocka, Khalid S. Mohammad, Mary R. Saunders, Hongge Li et al.
Journal of Clinical Investigation

Inflammation Controls B Lymphopoiesis by Regulating Chemokine CXCL12 Expression
Authors: Yoshihiro Ueda, Kaiyong Yang, Sandra J. Foster, Motonari Kondo, Garnett Kelsoe
The Journal of Experimental Medicine