Human/Mouse FosB/G0S3 Antibody

Catalog #: MAB2214 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2214
MAB2214-SP

Detection of Human and Mouse FosB/G0S3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FosB/G0S3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse FosB in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 269928

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FosB
Met1-Leu338
Accession # P53539

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse FosB/G0S3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with TPA for 2 hours and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with serum for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse FosB/G0S3 Monoclonal Antibody (Catalog # MAB2214), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FosB/G0S3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FosB/G0S3

FosB (FBJ murine osteoscarcoma viral oncogene homolog B) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. AP-1 activity is important for gene transcription in a wide variety of cellular processes.

Long Name

FBJ Murine Osteosarcoma Viral Oncogene Homolog B

Entrez Gene IDs

2354 (Human); 14282 (Mouse)

Alternate Names

activator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0S3; G0S3G0/G1 switch regulatory protein 3; GOS3; GOSB; MGC42291; oncogene FOS-B; protein fosB