Human/Mouse GLI-3 Biotinylated Antibody

Catalog #: BAF3690 Datasheet / COA / SDS

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BAF3690

All GLI-3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-3 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse GLI-3 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GLI‑3
Recombinant Mouse GLI‑3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-3

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Long Name

GLI-Kruppel family member GLI3

Entrez Gene IDs

2737 (Human); 14634 (Mouse); 140588 (Rat)

Alternate Names

ACLS; ACLSPAPAPHS; ADD; Bph; GCPS; GLI family zinc finger 3; GLI3 form of 190 kDa; GLI3 full length protein; GLI3; GLI-3; GLI3-190; GLI3FL; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; Greig cephalopolysyndactyly syndrome; oncogene GLI3; PAP-A; PAPA1; PAPB; PAPBDNA-binding protein; Pdn; PHS; PPDIV; Xt; zinc finger protein GLI3