Human/Mouse Kir2.1 Antibody Summary
Asn352-Ile427
Accession # P63252
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Kir2.1 by Western Blot. Western blot shows lysates of Human heart ventricle tissue and human lung tissue. PVDF membrane was probed with 3 µg/mL of Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Kir2.1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Kir2.1 in Human Heart. Kir2.1 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated discs. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Kir2.1 in Mouse Heart. Kir2.1 was detected in perfusion fixed frozen sections of mouse heart using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated discs. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Kir2.1 in Human Cardiomyocytes. Kir2.1 was detected in immersion fixed human cardiomyocytes using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kir2.1
Human Inward Rectifier Potassium Channel 2 (KIR2.1), also known as KCNJ2, is a 427 amino acid (aa) integral membrane protein with 98% homology with mouse and rat KIR2.1. This potassium channel preferentially allows potassium to flow into the cell, which is thought to generate action potential waveform and excitability in neuronal and muscle tissues. Mutations in the KIR2.1 gene are associated with Andersen syndrome, with associated paralysis, cardiac arrhythmias and dysmorphic features. KIR2.1 is widely expressed, notably in heart, placenta, lung, skeletal muscle, kidney and spleen. Expression of KIR2.1 is necessary for development of mature cardiomyocytes from mouse embryonic stem cells.
Product Datasheets
Citations for Human/Mouse Kir2.1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters
Authors: W Yu, Z Wang, X Yu, Y Zhao, Z Xie, K Zhang, Z Chi, S Chen, T Xu, D Jiang, X Guo, M Li, J Zhang, H Fang, D Yang, Y Guo, X Yang, X Zhang, Y Wu, W Yang, D Wang
Nature Communications, 2022-06-21;13(1):3544.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Cardiomyocytes Derived from Human CardiopoieticAmniotic Fluids
Authors: A Di Baldass, MA D'Amico, P Izzicupo, G Gaggi, S Guarnieri, MA Mariggiò, I Antonucci, B Corneo, D Sirabella, L Stuppia, B Ghinassi
Sci Rep, 2018-08-13;8(1):12028.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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