Human/Mouse LRRC4 Antibody

Catalog #: MAB4995 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4995
MAB4995-SP

Detection of Human and Mouse LRRC4 by Western Blot.

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All LRRC4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse LRRC4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse LRRC4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRRC3, rhLRRC4B, or rhLRRC32 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 701424

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LRRC4
Ala39-Lys527
Accession # Q9HBW1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse LRRC4 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LRRC4 Monoclonal Antibody (Catalog # MAB4995) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRRC4 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC4

LRRC4 (Leucine rich repeat/LRR-containing protein 4), also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma-associated gene 14) is a 55 kDa (predicted, unglycosylated) type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules (1, 2). Human LRRC4 cDNA encodes 653 amino acids (aa) that include a 38 aa signal sequence, a 489 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 105 aa cytoplasmic domain. The ECD contains nine LRRs (aa 74‑288), a C2 type Ig like domain (aa 354‑440), and a Thr-rich segment (aa 455‑526). Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54‑55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands (1). LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 on the dendritic surface of synaptic neurons (2-4). It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface (3, 5). It suppresses proliferation by downregulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase (1, 2, 6-8). It is thus considered a tumor suppressor protein and is often downregulated in brain tumors, particularly gliomas (1, 2, 6). Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation (1, 4, 9). Addition of soluable LRRC4 to cultured neurons reduces excitatory synapse formation (3).

References

Woo, J. et al. (2009) Mol. Cell. Neurosci. 42:1.
Zhang, Q. et al. (2005) FEBS Lett. 579:3674.
Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
Wu, M. et al. (2007) Acta Biochim Biophys Sin (Shanghai) 39:731.
Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14801.
Wu, M. et al. (2006) Mol. Biol. Cell 17:3534.
Wu, M. et al. (2008) J. Cell. Biochem. 103:245.
Wu, M. et al. (2008) J. Cell. Physiol. 214:65.
Zhang, W. et al. (2008) Genes Brain Behav. 7:385.

Long Name

Leucine-rich Repeat Containing 4

Entrez Gene IDs

64101 (Human); 192198 (Mouse); 641521 (Rat)

Alternate Names

BAG; Brain Tumor Associated Protein LRRC4; Brain Tumor-Associated Protein BAG; Leucine Rich Repeat Containing 4; Leucine-Rich Repeat-Containing 4; Leucine-Rich Repeat-Containing Protein 4; LRRC4; NAG14; Nasopharyngeal Carcinoma-Associated Gene 14 Protein; Netrin-G2 Ligand; NGL-2