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Human/Mouse PSMB7 Antibody

Catalog #: MAB7590
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7590
MAB7590-SP
Detection of Human and Mouse PSMB7 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PSMB7 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human PSMB7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PSMB10 is observed.
Source
Monoclonal Mouse IgG1 Clone # 787807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PSMB7
Thr44-Ser277
Accession # Q99436
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse PSMB7 antibody by Western Blot. View Larger

Detection of Human and Mouse PSMB7 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, AR42J human acinar pancreatic cancer cell line, HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, MCF-7 human breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human PSMB7 Monoclonal Antibody (Catalog # MAB7590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PSMB7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMB7

PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Active human PSMB7 shares 97% aa sequence identity with mouse and rat PSMB7. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.

Long Name
Proteasome (Prosome, Macropain) Subunit, beta Type, 7
Entrez Gene IDs
5695 (Human); 19177 (Mouse); 85492 (Rat)
Alternate Names
Macropain Chain Z; Proteasome Subunit Z; PSMB7; Z

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Citations for Human/Mouse PSMB7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation
    Authors: J Du, S Babik, Y Li, KK Deol, SJ Eyles, J Fejzo, M Tonelli, E Strieter
    Elife, 2022-04-22;11(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote Degradation
    Authors: Kirandeep K. Deol, Sean O. Crowe, Jiale Du, Heather A. Bisbee, Robert G. Guenette, Eric R. Strieter
    Molecular Cell

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