Human/Mouse/Rat HSP70/HSPA1A Antibody

Name: Human/Mouse/Rat HSP70/HSPA1A Antibody
Brand: R&D Systems
SKU: A-400

Catalog #: A-400 Datasheet / COA / SDS

Discontinued Product

A-400 has been discontinued.

View all HSP70/HSPA1A products.

Detection of Human HSP70/HSPA1A by Western Blot.

1 Image

All HSP70/HSPA1A Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSP70/HSPA1A Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects the induced form of human and mouse HSP70/HSPA1A in Western blots. No cross reactivity with HSC70 (HSP73) is observed.

Source

Monoclonal Mouse IgG_2A Clone # 242707

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HSP70/HSPA1A
Met1-Asp641

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human HSP70/HSPA1A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # A-400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP70/HSPA1A at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: HSP70/HSPA1A

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions, but are dramatically up-regulated in response to cellular stress. HSP70 (also known as HSPA1A, HSP70-1, and HSP72) is a 72 kDa member of the heat shock protein 70 family of proteins. HSP70 is required to target some misfolded proteins to the Ubiquitin E3 ligase CHIP (also known as Stub1) for subsequent ubiquitination.

Long Name

Heat Shock Protein 70

Entrez Gene IDs

3303 (Human); 15511 (Mouse); 24472 (Rat)

Alternate Names

dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.