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Human/Mouse/Rat HSP70/HSPA1A Antibody

Catalog #: MAB1663
17 Citations 1 Review Datasheet / COA / SDS
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MAB1663
MAB1663-SP
Detection of Human and Mouse HSP70/ HSPA1A by Western Blot.
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Citations (17)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSP70/HSPA1A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects the induced form of human and mouse HSP70/HSPA1A in Western blots. In Western blots, no cross-reactivity with the constitutively expressed HSC70 (HSP73) is detected.
Source
Monoclonal Mouse IgG2A Clone # 242707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HSP70/HSPA1A
Met1-Asp641
Accession # NP_005336
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse HSP70/ HSPA1A antibody by Western Blot. View Larger

Detection of Human and Mouse HSP70/ HSPA1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry HSP70/HSPA1A antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HSP70/HSPA1A in Human Liver Cancer Tissue. HSP70/HSPA1A was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human HSP70/HSPA1A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human HSP70/HSPA1A by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse HSP70/HSPA1A by Western Blot View Larger

Detection of Mouse HSP70/HSPA1A by Western Blot KNK437 inhibition assay of cell cultures. (A) Viability test results of KNK437 (10, 50 and 100 μM) inhibition assay of mononuclear peripheral blood burst formation unit-erythroid (BFU-E) cultures, with and without EPO. (B) Viability test results of a KNK437 (50 and 100 μM) inhibition assay of CD34+ bone marrow BFU-E cultures, with and without EPO. (C) Viability test results of KNK437 treatment (50 μM) on HEL and Ba/F3 JAK2 V617F (BAF3MUT) cell lines. X axis: concentration (μM); Y axis: % of viability cells (viability test) or BFU-E (BFU-E cultures) (D) Molecular effects of KNK437 in HEL cell line Western blot of HSP90, HSP70, p-JAK2 (decreased with KNK437 treatment), p-ERK and p-P38. Actin was used as the housekeeping control. (E) Molecular effects of KNK437 in Ba/F3 cell line Western blot of JAK2, p-ERK, ERK, p-STAT5, STAT5 AND Actin, (F) Western blot of HSP90, HSP70, JAK2, STAT5 and p-STAT5. Expression levels of HSP70, JAK2 and p-STAT5 decreased after HSP70 siRNA interference on HEL cell line. Actin was used as the housekeeping control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24252366), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human HSP70/HSPA1A by Western Blot View Larger

Detection of Human HSP70/HSPA1A by Western Blot KNK437 inhibition assay of cell cultures. (A) Viability test results of KNK437 (10, 50 and 100 μM) inhibition assay of mononuclear peripheral blood burst formation unit-erythroid (BFU-E) cultures, with and without EPO. (B) Viability test results of a KNK437 (50 and 100 μM) inhibition assay of CD34+ bone marrow BFU-E cultures, with and without EPO. (C) Viability test results of KNK437 treatment (50 μM) on HEL and Ba/F3 JAK2 V617F (BAF3MUT) cell lines. X axis: concentration (μM); Y axis: % of viability cells (viability test) or BFU-E (BFU-E cultures) (D) Molecular effects of KNK437 in HEL cell line Western blot of HSP90, HSP70, p-JAK2 (decreased with KNK437 treatment), p-ERK and p-P38. Actin was used as the housekeeping control. (E) Molecular effects of KNK437 in Ba/F3 cell line Western blot of JAK2, p-ERK, ERK, p-STAT5, STAT5 AND Actin, (F) Western blot of HSP90, HSP70, JAK2, STAT5 and p-STAT5. Expression levels of HSP70, JAK2 and p-STAT5 decreased after HSP70 siRNA interference on HEL cell line. Actin was used as the housekeeping control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24252366), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP70/HSPA1A

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Long Name
Heat Shock Protein 70
Entrez Gene IDs
3303 (Human); 15511 (Mouse); 24472 (Rat)
Alternate Names
dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

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Citations for Human/Mouse/Rat HSP70/HSPA1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Small RNAs detected in exosomes derived from the MH7A synovial fibroblast cell line with TNF-? stimulation
    Authors: Y Takamura, W Aoki, A Satomura, S Shibasaki, M Ueda
    PLoS ONE, 2018-08-10;13(8):e0201851.
  2. The bacterial toxin ExoU requires a host trafficking chaperone for transportation and to induce necrosis
    Authors: Vincent Deruelle, Stéphanie Bouillot, Viviana Job, Emmanuel Taillebourg, Marie-Odile Fauvarque, Ina Attrée et al.
    Nature Communications
  3. Engineered Extracellular Vesicles with Compound-Induced Cargo Delivery to Solid Tumors
    Authors: Kim, R;Kim, JH;
    International journal of molecular sciences
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Extracellular Vesicles
    Applications: Western Blot
  4. Analyzing the interactome of human CK2 beta in prostate carcinoma cells reveals HSP70 ‐1 and Rho guanin nucleotide exchange factor 12 as novel interaction partners
    Authors: Anna Nickelsen, Claudia Götz, Florian Lenz, Karsten Niefind, Simone König, Joachim Jose
    FASEB BioAdvances
  5. MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
    Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
    International Journal of Molecular Sciences, 2022-01-08;23(2):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression
    Authors: R Sonntag, C Penners, M Kohlhepp, U Haas, D Lambertz, A Kroh, T Cramer, F Ticconi, IG Costa, F Tacke, N Gassler, C Trautwein, C Liedtke
    Cancers, 2021-11-13;13(22):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Heat Shock Proteins HSPA1 and HSP90AA1 Are Upregulated in Colorectal Polyps and Can Be Targeted in Cancer Cells by Anti-Inflammatory Oxicams with Arylpiperazine Pharmacophore and Benzoyl Moiety Substitutions at Thiazine Ring
    Authors: I Szczuka, J Wierzbicki, P Serek, BM Szcz??niak, M Krzystek-K
    Biomolecules, 2021-10-27;11(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. Mebendazole Mediates Proteasomal Degradation of GLI Transcription Factors in Acute Myeloid Leukemia
    Authors: Fabian Freisleben, Franziska Modemann, Jana Muschhammer, Hauke Stamm, Franziska Brauneck, Alexander Krispien et al.
    International Journal of Molecular Sciences
  9. Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis
    Authors: Nik Mohamed Kamal NNS, Awang RAR, Mohamad S, Shahidan WNS.
    Frontiers in Physiology
  10. Hsc70/Stub1 promotes the removal of individual oxidatively stressed peroxisomes
    Authors: BH Chen, YJ Chang, S Lin, WY Yang
    Nat Commun, 2020-10-19;11(1):5267.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020-04-04;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray
  12. A novel anti-HER2 anthracycline-based antibody-drug conjugate induces adaptive anti-tumor immunity and potentiates PD-1 blockade in breast cancer
    Authors: Lucia D’Amico, Ulrike Menzel, Michael Prummer, Philipp Müller, Mélanie Buchi, Abhishek Kashyap et al.
    Journal for ImmunoTherapy of Cancer
  13. Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
    Authors: Guangxin Li
    Am J Transl Res, 2016-10-15;8(10):4184-4194.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Western Blot
  14. OLA1 protects cells in heat shock by stabilizing HSP70
    Authors: R-F Mao, V Rubio, H Chen, L Bai, O C Mansour, Z-Z Shi
    Cell Death & Disease
  15. Motherʼs Milk-Induced Hsp70 Expression Preserves Intestinal Epithelial Barrier Function in an Immature Rat Pup Model
    Authors: JENNIFER L. LIEDEL, YUEE GUO, YUEYUE YU, SHENG-RU SHIOU, SANGZI CHEN, ELAINE O. PETROF et al.
    Pediatric Research
  16. Proteomic Analysis of Heat Shock-Induced Protection in Acute Pancreatitis
    Authors: Vanessa Fétaud-Lapierre, Catherine M. Pastor, Annarita Farina, Denis F. Hochstrasser, Jean-Louis Frossard, Pierre Lescuyer
    Journal of Proteome Research
  17. Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo.
    Authors: Bhatnagar S, Shinagawa K, Castellino FJ, Schorey JS
    Blood, 2007-07-31;110(9):3234-44.
    Species: Human
    Sample Types: Exosomes
    Applications: Western Blot

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Human/Mouse/Rat HSP70/HSPA1A Antibody
By Anonymous on 08/19/2021
Application: IHC Sample Tested: Spinal cord Species: Mouse
Immunohistochemistry Human/Mouse/Rat HSP70/HSPA1A Antibody MAB1663