Human/Mouse/Rat p130Cas Antibody

Catalog #: MAB5730
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5730
MAB5730-SP

Detection of Human, Mouse, and Rat p130Cas by Western Blot.

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All p130Cas Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat p130Cas Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human p130Cas in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ajuba is observed.

Source

Monoclonal Mouse IgG₁ Clone # 685718

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human p130Cas
Met66-Val412
Accession # P56945

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat p130Cas by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human p130Cas Monoclonal Antibody (Catalog # MAB5730) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p130Cas at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p130Cas

p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105-130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Pro‑rich region (aa 74-87), at least 10 phosphotyrosine (aa 128-410) and four phosphoserine (aa 134-639) sites, an SH3 binding motif (aa 635-643) and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22 aa substitution for aa 1-4, an alternate start site at Met30, a deletion of aa 2-211, and a four aa substitution for aa 1-4 with a deletion of aa 65-212. Over aa 66-412, human p130Cas shares 91% aa identity with mouse p130Cas.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Entrez Gene IDs

9564 (Human); 12927 (Mouse); 25414 (Rat)

Alternate Names

BCAR1; breast cancer estrogen resistance 1; Cas scaffolding protein family member 1; CAS1; CASFLJ45059; CASS1; CASS1CRKASbreast cancer estrogen resistance protein 1; CRKAS; Crk-associated substrate p130Cas; CRK-associated substrate; FLJ12176; p130Cas

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citation for Human/Mouse/Rat p130Cas Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Conditional deletion of CD98hc inhibits osteoclast development
Authors: Hideki Tsumura, Morihiro Ito, Masamichi Takami, Miyuki Arai, Xiao-Kang Li, Toshio Hamatani et al.
Biochemistry and Biophysics Reports