Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antibody

Name: Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antibody
Brand: R&D Systems
SKU: AF2990
Rating: 3.8 (4 reviews)

Catalog #: AF2990 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2990
AF2990-SP

Detection of Rat and Human Phospho-MKK4 (S257/T261) by Western Blot.

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Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MKK4 when dually phosphorylated at sites corresponding to S257 and T261 of human MKK4.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human MKK4 S257/T261 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

3-15 µg/mL

Immersion fixed HeLa Human Cervical Epithelial Carcinoma Cells (Positive control) and HeLa Human Cervical Epithelial Carcinoma Cells treated with CIP (Calf Intestinal Phosphatase) (Negative Control)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat and Human Phospho-MKK4 (S257/T261) by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 300 mM sorbitol for 30 minutes and HepG2 human hepatocellular carcinoma cell line untreated or treated with 10 ng/mL Recombinant Human IL-1 beta (201-LB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2990), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-MKK4 (S257/T261) at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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Detection of MKK4 in HeLa Cells (Positive Control) and HeLa Cells treated with CIP (Calf Intestinal Phosphatase) (Negative Control). MKK4 was detected in immersion fixed HeLa Human Cervical Epithelial Carcinoma Cells (Positive control) and absent in HeLa Human Cervical Epithelial Carcinoma Cells treated with CIP (Calf Intestinal Phosphatase) (Negative Control) using Rabbit Anti-Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2990) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK4

Mitogen-activated protein kinase kinase 4 (MKK4 or MAP2K4), also known as SAPK/ERK kinase 1 (SEK1) and Jun kinase kinase 1 (JNKK1), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser257 and Thr261 by several upstream MAPK kinase kinases (MAP3Ks). MKK4 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 4

Entrez Gene IDs

6416 (Human)

Alternate Names

c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC 2.7.12.2

Product Datasheets

Product Datasheet

COA

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Product Specific Notices

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This product is sold pursuant to a license from the University of Massachusetts, and the University of Massachusetts reserves all other rights to use these reagents. For information on purchasing a license for uses other than research, please contact the University of Massachusetts, Office of Technology Management, 333 South Street, Suite 400, Shrewsbury, Massachusetts, 01545.