Human/Mouse/Rat RNF2 Antibody

Catalog #: AF6065 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6065
AF6065-SP

Detection of Human, Mouse, and Rat RNF2 by Western Blot.

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Human/Mouse/Rat RNF2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat RNF2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RNF2
aa 147-228
Accession # Q99496

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat RNF2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RNF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RNF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RNF2

RNF2 (RING [really interesting gene] finger protein 2/1B; also BAP-1, HIP-2 interacting protein 3 and DinG) is a 37 kDa member of the ring finger domain family of proteins. It is expressed in embryonic tissue, and serves as an E3 ubiquitin ligase within a polycomb complex that acts on histone H2A. In effect, RNF2 contributes to gene repression by blocking RNA pol II advance along gene promoters. RNF2 forms homodimers, and interacts with over a dozen proteins. Human RNF2 is 336 amino acids (aa) in length contains one Zn‑finger domain (aa 51‑91) and two phosphorylation sites at Ser168 and Ser203. There is one potential isoform that shows a deletion of aa 84‑155. Over aa 147‑228, human RNF2 shares 99% aa identity with mouse RNF2.

Long Name

Ring Finger Protein 2

Entrez Gene IDs

6045 (Human); 19821 (Mouse); 304850 (Rat)

Alternate Names

BAP-1; BAP1RING finger protein 1B; DING; DINGRING finger protein BAP-1; EC 6.3.2; HIPI3; HIPI3RING1BE3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; Protein DinG; ring finger protein 2HIP2-interacting protein 3; RING1B; RING2; RING2EC 6.3.2.-; RNF2