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Human MRP1 PE-conjugated Antibody

Catalog #: IC19291P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC19291P
Detection of MRP1 in H69AR Human Cell Line by Flow Cytometry.
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Citations (2)
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Human MRP1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MRP1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # QCRL
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
H69AR human small cell lung cancer cell line membrane extracts
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of MRP1 antibody in H69AR Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MRP1 in H69AR Human Cell Line by Flow Cytometry. H69AR human small cell lung cancer cell line was stained with Mouse Anti-Human MRP1 PE-conjugated Monoclonal Antibody (Catalog # IC19291P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MRP1

MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.

Long Name
Multi-drug Resistance Protein 1
Entrez Gene IDs
4363 (Human); 17250 (Mouse); 24565 (Rat)
Alternate Names
ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 3.6.3.44; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein

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Citations for Human MRP1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance
    Authors: M Colombo, S Garavelli, M Mazzola, N Platonova, D Giannandre, R Colella, L Apicella, M Lancellott, E Lesma, S Ancona, MT Palano, M Barbieri, E Taiana, E Lazzari, A Basile, M Turrini, A Pistocchi, A Neri, R Chiaramont
    Haematologica, 2019-10-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Multidrug-resistance-associated protein plays a protective role in menadione-induced oxidative stress in endothelial cells.
    Authors: Takahashi K, Shibata T, Oba T, Ishikawa T, Yoshikawa M, Tatsunami R, Takahashi K, Tampo Y
    Life Sci., 2008-12-07;84(7):211-7.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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