Human NDRG1 Antibody

Catalog #: AF5209 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5209
AF5209-SP

Detection of Human NDRG1 by Western Blot.

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Human NDRG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NDRG1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human N-myc is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NDRG1
Met111-Asp267
Accession # Q92597

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NDRG1 by Western Blot. Western blot shows lysates of human liver tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NDRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NDRG1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NDRG1

NDRG1 (N-myc downstream-regulated gene 1; also DRG-1 and Cap43) is a 43 kDa member of the NDRG family of proteins. It is ubiquitously expressed, found in fibroblasts, endothelial cells, respiratory epithelium and prostate epithelium. NDRG1 is highly expressed in nonproliferating, differentiating tissue. Human NDRG1 is 394 amino acids (aa) in length. It contains an NDGR domain (aa 286‑316) plus three tandem 10 aa hydrophilic repeats (aa 339‑368). There are three potential isoform variants. Two involve alternate start sites at Met82 and Met286, while a third shows a four aa substitution for aa 1‑34. Over aa 11‑267, human NDRG1 is 95% aa identical to mouse NDRG1.

Long Name

N-myc Downstream Regulated Gene 1

Entrez Gene IDs

10397 (Human); 17988 (Mouse); 299923 (Rat)

Alternate Names

CAP43; CMT4D; Differentiation-related gene 1 protein; DRG1; DRG-1; DRG1HMSNL; GC4; HMSNL; NDR1; NDRG1; Nickel-specific induction protein Cap43; NMSL; N-myc downstream regulated 1; N-myc downstream-regulated gene 1 protein; protein NDRG1; protein regulated by oxygen-1; PROXY1; Reducing agents and tunicamycin-responsive protein; RIT42; RTP; RTPGC4; TARG1; TDD5; tunicamycin-responsive protein