Human Nesfatin-1/Nucleobindin-2 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Nesfatin-1/Nucleobindin-2
Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1- 24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.
Citations for Human Nesfatin-1/Nucleobindin-2 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Dynamics of Serum Inflammatory Markers and Adipokines in Patients: Implications for Monitoring Abnormal Body Weight: Preliminary Research
Authors: Zimowska, M;Rolbiecka, M;Antoniak-Pietrynczak, K;Jaskulak, M;Zorena, K;
Metabolites
Species: Human
Sample Types: Serum
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Nesfatin-1 in Human Milk and Its Association with Infant Anthropometry
Authors: KD Honoré, S Bruun, LN Jacobsen, M Domellöf, KF Michaelsen, S Husby, G Zachariass
Nutrients, 2022-12-30;15(1):.
Species: Human
Sample Types: Milk
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Whey Protein Supplementation Compared to Collagen Increases Blood Nesfatin Concentrations and Decreases Android Fat in Overweight Women: A Randomized Double-Blind Study
Authors: BM Giglio, RM Schincagli, AS da Silva, ICS Fazani, PA Monteiro, JF Mota, JP Cunha, C Pichard, GD Pimentel
Nutrients, 2019-09-02;11(9):.
Species: Human
Sample Types: Plasma
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Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis
Authors: C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein
Peptides, 2018-02-21;102(0):31-37.
Species: Human
Sample Types: Plasma
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Nesfatin-1/Nucleobindin-2 enhances cell migration, invasion, and epithelial-mesenchymal transition via LKB1/AMPK/TORC1/ZEB1 pathways in colon cancer
Oncotarget, 2016-05-24;7(21):31336-49.
Species: Human
Sample Types: Serum
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Nesfatin-1 stimulates fatty-acid oxidation by activating AMP-activated protein kinase in STZ-induced type 2 diabetic mice.
Authors: Dong J, Xu H, Xu H, Wang P, Cai G, Song H, Wang C, Dong Z, Ju Y, Jiang Z
PLoS ONE, 2013-12-31;8(12):e83397.
Species: Mouse
Sample Types: Plasma
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