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Human NG2/MCSP Antibody

Catalog #: MAB2585
15 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2585
MAB2585-SP
Detection of Human NG2/MCSP by Western Blot.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP Antibody Summary

Species Reactivity
Human
Specificity
Detects human NG2/MCSP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # LHM-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human melanoma cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
see below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NG2/MCSP antibody by Western Blot. View Larger

Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry NG2/MCSP antibody in Human Skin Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

NG2/MCSP in Human Skin Cancer Tissue. NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) using 10 µg/mL Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry View Larger

Detection of NG2/MCSP in A375 cells by Flow Cytometry A375 cells were stained with Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References
  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Human NG2/MCSP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. TORC1 Suppression Predicts Responsiveness to RAF and MEK Inhibition in BRAF- Mutant Melanoma
    Authors: Ryan B. Corcoran, Stephen Michael Rothenberg, Aaron N. Hata, Anthony C. Faber, Adriano Piris, Rosalynn M. Nazarian et al.
    Science Translational Medicine
  2. Limited extent and consequences of pancreatic SARS-CoV-2 infection
    Authors: Verena van der Heide, Sonia Jangra, Phillip Cohen, Raveen Rathnasinghe, Sadaf Aslam, Teresa Aydillo et al.
    Cell Reports
  3. Immunohistochemical and Ultrastructural Characterization of Telocytes in Normal and Diabetic Human Kidneys
    Authors: Valente, S;Villacampa Lahoz, M;Vasuri, F;Pasquinelli, G;
    Biomolecules
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
    Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
    Sci Adv, 2020-02-26;6(9):eaax3223.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  5. Amyloid-beta 1-40 is associated with alterations in NG2+ pericyte population ex�vivo and in�vitro
    Authors: N Schultz, K Brännström, E Byman, S Moussaud, HM Nielsen, A Olofsson, M Wennström
    Aging Cell, 2018-02-17;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
    Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
    Stem Cells Dev., 2017-05-09;0(0):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  7. Isolation and characterization of equine native MSC populations
    Authors: CL Esteves, TA Sheldrake, SP Mesquita, JJ Pesántez, T Menghini, L Dawson, B Péault, FX Donadeu
    Stem Cell Res Ther, 2017-04-18;8(1):80.
    Species: Equine
    Sample Types: Whole Tissue
    Applications: IHC
  8. Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro.
    Authors: Higgins S, Fillmore H, Ashkan K, Butt A, Pilkington G
    Anticancer Res, 2015-01-01;35(1):77-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Expression of the chondroitin sulphate proteoglycan, NG2, in paediatric brain tumors.
    Authors: Higgins S, Bolteus A, Donovan L, Hasegawa H, Doey L, Al Sarraj S, King A, Ashkan K, Roncaroli F, Fillmore H, Pilkington G
    Anticancer Res, 2014-12-01;34(12):6919-24.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  10. Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
    Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
    PLoS ONE, 2014-09-26;9(9):e107642.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Chondroitin sulfate proteoglycan CSPG4 as a novel hypoxia-sensitive marker in pancreatic tumors.
    Authors: Keleg S, Titov A, Heller A, Giese T, Tjaden C, Ahmad S, Gaida M, Bauer A, Werner J, Giese N
    PLoS ONE, 2014-06-16;9(6):e100178.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  12. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013-04-24;497(7449):369-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Two distinct types of blood vessels in clear cell renal cell carcinoma have contrasting prognostic implications.
    Authors: Yao X, Qian CN, Zhang ZF, Tan MH, Kort EJ, Yang XJ, Resau JH, Teh BT
    Clin. Cancer Res., 2007-01-01;13(1):161-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Mathematical-model-guided development of full-thickness epidermal equivalent
    Authors: Junichi Kumamoto, Shinobu Nakanishi, Mio Makita, Masaaki Uesaka, Yusuke Yasugahira, Yasuaki Kobayashi et al.
    Scientific Reports
  15. Capture and On-chip analysis of Melanoma Cells Using Tunable Surface Shear forces
    Authors: Simon Chang-Hao Tsao, Ramanathan Vaidyanathan, Shuvashis Dey, Laura G. Carrascosa, Christopher Christophi, Jonathan Cebon et al.
    Scientific Reports

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Reviews for Human NG2/MCSP Antibody

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Human NG2/MCSP Antibody
By Anonymous on 10/15/2021
Application: IHC Sample Tested: brain tumor Species: Human
Immunohistochemistry Human NG2/MCSP Antibody MAB2585
Human NG2/MCSP Antibody
By Anonymous on 07/15/2021
Application: IHC Sample Tested: White matter (brain) Species: Human
Immunohistochemistry Human NG2/MCSP Antibody MAB2585