Human NG2/MCSP Antibody

Catalog #: AF2585
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2585
AF2585-SP

Detection of Human NG2/MCSP by Western Blot.

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All NG2/MCSP Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP Antibody Summary

Species Reactivity

Human

Specificity

Detects human NG2/MCSP in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse CSPG4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NG2/MCSP
Ser1583-Ser2224
Accession # Q6UVK1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Knockout Validated

NG2/MCSP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NG2/MCSP knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human NG2/MCSP Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human NG2/MCSP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2 (neuron/glia-type 2 antigen; also MCSP/melanoma chondroitin sulfate proteoglycan) is a 250‑500 kDa type I integral membrane proteoglycan found on synantocytes (NG2+ glia), vascular pericytes, chondroblasts, macrophages and melanoma cells. It binds multiple components of the ECM, and serves as a ligand for alpha 3 beta 1 and alpha 4 beta 1 integrins. Mature human NG2 is 2293 amino acids (aa) in length. It contains a large 2195 aa extracellular region (aa 30‑2224) and a short 79 aa cytoplasmic tail. NG2 is variably glycanated. Without proteoglycan, NG2 is expressed as a 250 kDa glycoprotein. Juxtamembrane proteolysis generates a soluble ECD that noncovalently associates with the transmembrane fragment. Over aa 1583‑2224, human NG2 is 81% aa identical to both mouse and canine NG2.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Alternate Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NG2/MCSP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Proteomic profile of melanoma cell‐derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression
Authors: Monika Pietrowska, Aneta Zebrowska, Marta Gawin, Lukasz Marczak, Priyanka Sharma, Sujan Mondal et al.
Journal of Extracellular Vesicles
IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
Authors: Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschlager M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P
Oncogene, 2014-03-17;34(7):815-25.
Species: Human
Sample Types: Whole Tissue
Applications: IHC, IHC-Fr