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Human NG2/MCSP PE-conjugated Antibody

Catalog #: FAB2585P
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2585P
Detection of NG2/MCSP in A375 cells by Flow Cytometry
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NG2/MCSP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # LHM-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human melanoma cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
A375 and G361 human melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of NG2/MCSP in A375 cells by Flow Cytometry A375 cells were stained with Mouse Anti-Human NG2/MCSP PE‑conjugated Monoclonal Antibody (Catalog # FAB2585P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of NG2/MCSP antibody in G361 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP PE-conjugated Monoclonal Antibody (Catalog # FAB2585P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References
  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Human NG2/MCSP PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019-03-05;26(10):2566-2579.e10.
  2. Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head
    Authors: M Herrmann, M Hildebrand, U Menzel, N Fahy, M Alini, S Lang, L Benneker, S Verrier, MJ Stoddart, JJ Bara
    Int J Mol Sci, 2019-07-14;20(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.
    Authors: Yuan K, Orcholski M, Panaroni C, Shuffle E, Huang N, Jiang X, Tian W, Vladar E, Wang L, Nicolls M, Wu J, de Jesus Perez V
    Am J Pathol, 2014-11-06;185(1):69-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  4. Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB signaling pathway and proinflammatory response in human pericytes.
    Authors: Guijarro-Munoz I, Compte M, Alvarez-Cienfuegos A, Alvarez-Vallina L, Sanz L
    J Biol Chem, 2013-12-04;289(4):2457-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts.
    Authors: Orlova, Valeria, Drabsch, Yvette, Freund, Christia, Petrus-Reurer, Sandra, van den Hil, Francijn, Muenthaisong, Suchitra, Dijke, Peter Te, Mummery, Christin
    Arterioscler Thromb Vasc Biol, 2013-10-24;34(1):177-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
    Authors: Cheung C, Bernardo AS, Trotter MW
    Nat. Biotechnol., 2012-01-15;30(2):165-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2011-12-14;318(4):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Microvascular Networks From Endothelial Cells and Mesenchymal Stromal Cells From Adipose Tissue and Bone Marrow: A Comparison
    Authors: Karoline Pill, Johanna Melke, Severin Mühleder, Marianne Pultar, Sabrina Rohringer, Eleni Priglinger et al.
    Frontiers in Bioengineering and Biotechnology

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