Human NPDC-1 Antibody

Catalog #: AF5489 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5489
AF5489-SP

Detection of Human NPDC‑1 by Western Blot.

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Human NPDC-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NPDC-1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NPDC-1
Gly35-Asp181
Accession # Q9NQX5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NPDC‑1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NPDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NPDC-1 at approximately 40 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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NPDC‑1 in Human Brain. NPDC-1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human NPDC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles of neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPDC-1

NPDC-1 (Neural proliferation differentiation and control protein 1) is a 35 kDa member of the NPDC1/cab1 family of proteins. It is principally expressed in neurons, and serves as an anti-proliferation agent. It may accomplish this, in part, by binding to E2F-1 and blocking its protranscriptional activity. Mature human NPDC-1 is 291 amino acids (aa) in length. It is a type I transmembrane protein that contains an NLS (aa 107‑124), an HLH domain (aa 95‑128), a transmembrane domain (aa 182‑202), and a PEST degradation sequence (aa 269‑302). Phosphorylation promotes ubiquitination at this latter site. There are two splice variants. One shows a deletion of aa 242‑263, while another shows a nine aa substitution for the nine aa in positions 218‑226. Over aa 35‑181, human NPDC-1 shares 68% aa identity with mouse NPDC-1.

Long Name

Neural Proliferation, Differentiation and Control, 1

Entrez Gene IDs

56654 (Human); 18146 (Mouse); 296562 (Rat)

Alternate Names

CAB-; CAB1; CAB-1; CAB1CAB; DKFZp586J0523; neural proliferation differentiation and control protein 1; neural proliferation, differentiation and control, 1; NPDC1; NPDC-1