Human NT-ProANP DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Atrial Natriuretic Peptide/ANP
Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.
Citations for Human NT-ProANP DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Investigating the Effects of Atrial Natriuretic Peptide on the Maternal Endothelium to Determine Potential Implications for Preeclampsia
Authors: NK Binder, S Beard, N de Alwis, BR Fato, TV Nguyen, TJ Kaitu'u-Li, NJ Hannan
International Journal of Molecular Sciences, 2023-03-24;24(7):.
Species: Human
Sample Types: Plasma
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No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department
Authors: S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich
Scientific Reports, 2022-05-24;12(1):8733.
Species: Human
Sample Types: Serum
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The Effects of Short-Term Changes in Sodium Intake on Plasma Marinobufagenin Levels in Patients with Primary Salt-Sensitive and Salt-Insensitive Hypertension
Authors: K ?abno-Kirs, A Kujawa-Sze, A Wiecek, G Piecha
Nutrients, 2021-04-29;13(5):.
Species: Human
Sample Types: Serum
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Aberrant pro-atrial natriuretic peptide/corin/natriuretic peptide receptor signaling is present in maternal vascular endothelium in preeclampsia
Authors: Y Gu, D Thompson, J Xu, DF Lewis, JA Morgan, DB Cooper, CE McCathran, Y Wang
Pregnancy Hypertens, 2017-12-07;11(0):1-6.
Species: Human
Sample Types: Plasma
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