Human NT-ProANP Quantikine ELISA Kit

Catalog #: DANP00
4 Citations Datasheet / COA / SDS

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DANP00

Control Products Available

QC227: Quantikine Immunoassay Control Set 905 for Human NT-ProANP

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All Atrial Natriuretic Peptide/ANP ELISAs

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Citations (4)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human NT-ProANP Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.146 ng/mL

Assay Range

0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human NT-ProANP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.71	6.04	11.1	1.81	5.95	11.5
Standard Deviation	0.069	0.224	0.583	0.129	0.426	0.842
CV%	4	3.7	5.3	7.1	7.2	7.3

Recovery

The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	104	87-114
EDTA Plasma (n=4)	102	84-120
Heparin Plasma (n=4)	98	84-111
Serum (n=4)	108	95-122

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human NT-ProANP ELISA Standard Curve

ELISA

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Endothelin Induces Expression of Atrial Natriuretic Peptide in iPSC-derived Cardiomyoctyes. JOY6 human iPSCs were differentiated into beating cardiomyocytes. After 21 days of differentiation cardiomyocytes were treated with 5 nM of Endothelin-1 (Catalog # 1160) for 25.5 hours to induce cardiac hypertrophy. The level of secreted Pro-Atrial Natriuretic peptide (ANP), which is known to be elevated during cardiac hypertrophy, was assessed using the Human NT-ProANP Quantikine® ELISA Kit (Catalog # DANP00). As expected, detection of Pro-ANP was increased in Endothelin-1 treated cells.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Atrial Natriuretic Peptide/ANP

Atrial Natriuretic peptide (ANP) is synthesized as a 151 aa preproprotein. The proprotein contains cardiodilatin (aa 26 - 92) and atrial natriuretic factor (ANF; aa 124 - 151) which become active after processing by the serine protease corin. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. ANF circulates in the plasma, eliciting natriuretic, diuretic, vasorelaxant and antimitogenic effects directed toward reduction of body fluid and blood pressure regulation. ANF acts through the cGMP-coupled NP receptor A. Human ANP and ANF share 84% and 96% aa identity with mouse ANP and ANF, respectively.

Entrez Gene IDs:

4878 (Human); 230899 (Mouse); 24602 (Rat)

Alternate Names:

ANF; ANP; ANPatriopeptin; ATFB6; atrial natriuretic factor; Atrial Natriuretic Peptide; Cardionatrin; CDD-ANF; natriuretic peptide A; natriuretic peptide precursor A; NPPA; PND; PNDcardionatrin; prepronatriodilatin

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human NT-ProANP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Serum Biomarkers and Their Association with Myocardial Function and Exercise Capacity in Cardiac Transthyretin Amyloidosis
Authors: Nieto-Roca, L;Camblor Blasco, A;Devesa, A;Gómez-Talavera, S;Balaguer-Germán, J;Lumpuy-Castillo, J;Pello, AM;Dhier, LM;Lapeña, G;Jiménez, LL;Lorenzo, Ó;Tuñón, J;Ibáñez, B;Aceña, Á;
Journal of cardiovascular development and disease
Species: Human
Sample Types: Plasma
Dapagliflozin Improved Cardiac Function and Structure in Diabetic Patients with Preserved Ejection Fraction: Results of a Single Centre, Observational Prospective Study
Authors: Cortés, M;Lorenzo, O;Lumpuy-Castillo, J;Martínez-Albaladejo, S;Taibo-Urquía, M;Pello, AM;Bollas, AJ;Orejas, M;Navas, MÁ;Macia, E;Martínez, ME;Rueda, A;Tuñón, J;
Journal of clinical medicine
Species: Human
Sample Types: Plasma, Serum, Urine
Associations of atrial natriuretic peptide with measures of insulin and adipose depots
Authors: CA Couch, LA Fowler, V Parcha, P Arora, BA Gower
Physiological Reports, 2023-03-01;11(5):e15625.
Species: Human
Sample Types: Serum
Associations among NPPA gene polymorphisms, serum ANP levels, and hypertension in the Chinese Han population
Authors: H Zhang, X Mo, Z Zhou, Z Zhu, X HuangFu, T Xu, A Wang, Z Guo, Y Zhang
J Hum Hypertens, 2019-07-24;0(0):.
Species: Human
Sample Types: Serum