Human p16INK4a / CDKN2A Antibody Summary
Glu2-Asp156
Accession # P42771
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human p16INK4a/CDKN2A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, and Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p16INK4a/CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p16INK4a/CDKN2A at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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p16INK4a / CDKN2A in HeLa Human Cell Line. p16INK4a / CDKN2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human p16INK4a / CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human p16INK4a / CDKN2A by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p16INK4a / CDKN2A at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human p16INK4a / CDKN2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5779) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p16INK4a / CDKN2A
p16INK4a (16 kDa Inhibitor of CDK4-a; also MTS1, CDK41 and CDKN2) is a 16 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is widely expressed (although not in skeletal muscle) and serves as a negative regulator of cell proliferation. It does so by associating with CDK4 or 6, thereby blocking cyclin binding and subsequent Ser/Thr kinase activity. Human p16INK4a is 156 amino acids (aa) in length. It contains four “L” shaped ankyrin repeats
(aa 11‑139) that interact with cyclin. There are at least two splice variants for p16INK4a. One is termed p12 and shows a 65 aa substitution for aa 52‑156; the other simply shows an alternate start site at Met52. Full‑length human p16INK4a shares 63% aa identity with mouse p16INK4a.
Product Datasheets
Citations for Human p16INK4a / CDKN2A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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miR-1468-3p Promotes Aging-Related Cardiac Fibrosis
Authors: Ruizhu Lin, Lea Rahtu-Korpela, Johanna Magga, Johanna Ulvila, Julia Swan, Anna Kemppi et al.
Molecular Therapy - Nucleic Acids
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Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells
Authors: Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang
International Journal of Oncology
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Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro
Authors: Tom Zimmermann, Michaela Pommer, Viola Kluge, Chafia Chiheb, Susanne Muehlich, Anja-Katrin Bosserhoff
Cells
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Increased post-mitotic senescence in aged human neurons is a pathological feature of Alzheimer's disease
Authors: JR Herdy, L Traxler, RK Agarwal, L Karbacher, JCM Schlachetz, L Boehnke, D Zangwill, D Galasko, CK Glass, J Mertens, FH Gage
Cell Stem Cell, 2022-12-01;29(12):1637-1652.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Simple Western -
TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence
Authors: V Farfariell, DV Gordienko, L Mesilmany, Y Touil, E Germain, I Fliniaux, E Desruelles, D Gkika, M Roudbaraki, G Shapovalov, L Noyer, M Lebas, L Allart, N Zienthal-G, O Iamshanova, F Bonardi, M Figeac, W Laine, J Kluza, P Marchetti, B Quesnel, D Metzger, D Bernard, JB Parys, L Lemonnier, N Prevarskay
Nature Communications, 2022-02-17;13(1):956.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bacterial genotoxins induce T�cell senescence
Authors: SL Mathiasen, L Gall-Mas, IS Pateras, SDP Theodorou, MRJ Namini, MB Hansen, OCB Martin, CK Vadivel, K Ntostoglou, D Butter, M Givskov, C Geisler, AN Akbar, VG Gorgoulis, T Frisan, N Ødum, T Krejsgaard
Cell Reports, 2021-06-08;35(10):109220.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PRMT5 inhibition disrupts splicing and stemness in glioblastoma
Authors: P Sachamitr, JC Ho, FE Ciamponi, W Ba-Alawi, FJ Coutinho, P Guilhamon, MM Kushida, FMG Cavalli, L Lee, N Rastegar, V Vu, M Sánchez-Os, J Coulombe-H, E Kanshin, H Whetstone, M Durand, P Thibault, K Hart, M Mangos, J Veyhl, W Chen, N Tran, BC Duong, AM Aman, X Che, X Lan, O Whitley, O Zaslaver, D Barsyte-Lo, LM Richards, I Restall, A Caudy, HL Röst, ZQ Bonday, M Bernstein, S Das, MD Cusimano, J Spears, GD Bader, TJ Pugh, M Tyers, M Lupien, B Haibe-Kain, H Artee Luch, S Weiss, KB Massirer, P Prinos, CH Arrowsmith, PB Dirks
Nature Communications, 2021-02-12;12(1):979.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule
Authors: P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts
Cell Rep, 2019-12-10;29(11):3488-3505.e9.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Exposure of human melanocytes to UVB twice and subsequent incubation leads to cellular senescence and senescence-associated pigmentation through the prolonged p53 expression
Authors: SY Choi, BH Bin, W Kim, E Lee, TR Lee, EG Cho
J. Dermatol. Sci., 2018-03-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MAX inactivation is an early event in GIST development that regulates p16 and cell proliferation
Authors: IM Schaefer, Y Wang, CW Liang, N Bahri, A Quattrone, L Doyle, A Mariño-Enr, A Lauria, M Zhu, M Debiec-Ryc, S Grunewald, JF Hechtman, A Dufresne, CR Antonescu, C Beadling, ET Sicinska, M van de Rij, GD Demetri, M Ladanyi, CL Corless, MC Heinrich, CP Raut, S Bauer, JA Fletcher
Nat Commun, 2017-03-08;8(0):14674.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes
Authors: Taewon Jin
Biochem Biophys Res Commun, 2016-06-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of heparanase by HPV E6 oncogene in head and neck squamous cell carcinoma.
Authors: Hirshoren N, Bulvik R, Neuman T, Rubinstein A, Meirovitz A, Elkin M
J Cell Mol Med, 2013-11-28;18(1):181-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes
Authors: Rachael E. Schwartz, Maxim N. Shokhirev, Leonardo R. Andrade, J. Silvio Gutkind, Ramiro Iglesias-Bartolome, Gerald S. Shadel
Aging (Albany NY)
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Human p16INK4a/CDKN2A Antibody
Ref:AF5779 R et D Systems
Efficacité validée dans conditions suivantes:
Dilution anticorps p16= 1/10è (0,2mg/ml solution stock)
[HEK]= 0,65mg/ml
Anticorps 2aire goat prêt à l’emploi
5% non fat milk as blocking agent for 1 hour and overnight incubation with primary antibody at 1:3000. Specific band at 16kDa as expected, but also band at 25 KDa.
Cells at passage 2 had low p16 expression, while at passage 6 expression was increased as expected. Loading: p2,p6-p2,p6-p2,p6-p2,p6 of MSCs from 4 different individuals.
