Human p67phox Antibody

Catalog #: AF7830 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7830
AF7830-SP

Detection of Human p67phox by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human p67phox Antibody Summary

Species Reactivity

Human

Specificity

Detects human p67phox in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human p67phox
Lys355-Val526 (His389Gln)
Accession # P19878

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human p67phox by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and human immature dendritic cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human p67phox Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7830) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for p67phox at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p67phox

P67phox (67 kDa phagocyte oxidase; also NCF2 and NADPH oxidase activator 2) is a 67-70 kDa cytosolic member of the NCF2/NOXA1 family of proteins. It is expressed in multiple cell types, including B cells, endothelial cells, phagocytic cells such as neutrophils and macrophages and, to some degree, fibroblasts. Upon phagocytosis, phagocytes generate superoxide for the purpose of killing ingested microbes. This occurs through the formation and activation of a complex termed NADPH oxidase, which consists of membrane-associated cytochrome b558, plus Rac GTPase and three SH3 domain-containing components, p47phox and a p67phox:p40-phox dimer. Human p67phox is 526 amino acids (aa) in length. It contains two TPR repeats that participate in scaffold formation (aa 11-98), an SH3 domain (aa 240-299), an OPR motif that regulates PB1 interactions (aa 351-429), and a C-terminal SH3 domain (aa 457-516). There is one splice variant that shows a deletion of aa 231-293, and multiple point mutations along the length of the molecule. Over aa 355-526, human p67phox shares 76% aa identity with mouse p67phox.

Entrez Gene IDs

4688 (Human); 17970 (Mouse); 364018 (Rat)

Alternate Names

autosomal 2; NADPH oxidase activator 2; NCF2; NCF-2; neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal2); neutrophil cytosolic factor 2; NOXA2; NOXA2FLJ93058; p67phox; p67-phox